KeyGenNinja.com | 1 Catalog

KeyGenNinja.com | 1 Catalog

KeyGenNinja.com | 1 Catalog

KeyGenNinja.com | 1 Catalog

BigDye™ Terminator v Cycle Sequencing Kit

For Use With (Equipment): Genetic Analyzer, xl Genetic Analyzer, xl DNA Analyzer, GeneAmp , Veriti Thermal Cycler, Genetic Analyzer, DNA Analyzer, xL Genetic Analyzer, Genetic AnalyzerDescription:Cycle Sequencing Kit, 25, rxns, Includes 10 x 20mL tubes of BigDye™ Terminator v Ready Reaction Mix, 4 tubes M13 () Primer, 4 tubes pGEM Control DNA, 1 x mL tube of 5X Sequencing Buffer (may be stored at 4°C)Format:8-well strips, strip wells, well plate, well plateIncludes:10 x 20mL tubes of BigDye™ Terminator v Ready Reaction Mix, 4 tubes M13 () Primer, 4 tubes pGEM Control DNA, 1 x mL tube of 5X Sequencing Buffer (may be stored at 4°C)Recommended Applications:Standard SequencingTechnique:Fluorescent Dye Terminator SequencingTemplate Compatibility:BAC DNA, Plasmid DNA (≤15Kb), PCR Amplicons, Single Stranded DNAProduct Line:BigDye® Terminator, BigDye™
Источник: [manicapital.com]
, KeyGenNinja.com | 1 Catalog

Overview

mTeSR™1, now manufactured under cGMP, is the most widely published feeder-free cell culture medium for human embryonic stem cells (ES cells) and induced pluripotent stem cells (iPS cells), with established protocols for applications ranging from derivation to differentiation. It has been used to successfully maintain thousands of ES and iPS cell lines in over 50 countries, and has supported top pluripotent stem cell publications and researchers. mTeSR™1 is a highly specialized, serum-free and complete cell culture medium. With pre-screened raw materials that ensure batch-to-batch consistency and robust feeder-free protocols for ES and iPS cell culture, mTeSR™1 provides more consistent cultures with homogeneous, undifferentiated phenotypes.

mTeSR™1 is manufactured under relevant cGMPs, ensuring the highest quality and consistency for reproducible results.

To request a Letter of Authorization (LOA) for mTeSR’s Drug Master File, click here.

Components:

  • mTeSR™1 Complete Kit (Catalog #)
    • mTeSR™1 Basal Medium, mL
    • mTeSR™1 5X Supplement, mL
  • mTeSR™1 Complete Kit, 1 L (Catalog #)
    • mTeSR™1 Basal Medium, mL
    • mTeSR™1 5X Supplement, mL, 2 Bottles
  • mTeSR™1 Complete Kit, 10 Pack (Catalog #)
    • mTeSR™1 Basal Medium, mL, 10 Bottles
    • mTeSR™1 5X Supplement, mL, 10 Bottles
  • mTeSR™1 Complete Kit, 25 Pack (Catalog #)
    • mTeSR™1 Basal Medium, mL, 25 Bottles
    • mTeSR™1 5X Supplement, mL, 25 Bottles

Subtype:

Specialized Media

Cell Type:

Pluripotent Stem Cells

Application:

Cell Culture; Expansion; Maintenance

Area of Interest:

Stem Cell Biology

Источник: [manicapital.com]
KeyGenNinja.com | 1 Catalog

Step 1: Create Your Catalog File

In this step, you will create a catalog file that contains the metadata (title, length, release year, etc.) for your app's media content (movies, TV shows, specials, mini-series, and extras). The catalog file must follow the Catalog Data Format (CDF) schema, which requires you to follow a specific XML structure for describing your media.

Here is a video that will walk you through the process. For more details, continue reading the page below.

Creating Your Catalog File

Use an automated process to create the file by developing programmatic scripts that pull the information from your database and transform the data to a CDF-compliant catalog file.

In general, to create the file, you will need to first familiarize yourself with XML and its concepts as they relate to the catalog XSL schema — see the CDF Overview. You'll then download the CDF schema XSD file to understand the logic and rules at a more granular level. You can also use the code samples shown in the Element web pages or the CDF examples ZIP as a starting point. As you create the file, consult the CDF reference documentation.

Note: You should create your catalog file programmatically rather than manually. If for some reason you must manually adjust the file, such as for a one-time emergency fix, do not use Microsoft Word or some other rich-text editor. Use a text editor (for example, Visual Studio Code, Oxygen XML, Sublime Text, or even Notepad++).

An Example Catalog File

The following example shows a simple catalog file that contains only one item (a movie), and uses only required elements.

Note: We strongly recommend that you provide more details about each work than this, as this will potentially lead to higher quality feed and better matching. This limited example, however, is useful in showing the most basic catalog file structure. Download the manicapital.com file for larger and more comprehensive examples.

General Catalog File Structure

This section provides an overview of the structure of the CDF schema and its elements. Readers should have a good understanding of XML.

Note: This page is intended as a general overview of the catalog format, including its major elements and how they fit together. We don't cover the use of all possible elements here; for that, see the Catalog Data Format (CDF) Schema or the XSD schema file itself.

The top-level outline of a catalog file can be represented as:

Catalog (required) is the root element of all catalog files.

Partner (required) identifies you, the content provider.

Works (required) contains the bulk of the file: all of the movies and TV shows in your library, as much information as you choose to provide about each entry, and the offers that enable the user to view them.

The element can contain any number of child elements, each representing an individual work such as a movie or a TV show. If the element contains no child elements, we infer that all of your content is no longer available and we will remove it from our index.

The most common work type elements are as follows:

ElementDescription
Represents a feature-length film, but can also be used for short films.
A single episode of a , normally also associated with a .
One of the basic work types, a is a single season of a .
A televised series made up of seasons and episodes.
Televised events that don't belong to the traditional show-season-episode television hierarchy.
A clip or trailer that can be a standalone work or, more commonly, can be associated with another work (either external or in your catalog).

Movies are standalone elements. TV shows, TV seasons, and TV episodes are separate elements that are tied together by IDs, as are mini-series and mini-series episodes. TV specials and extras can be standalone elements, but they can also be tied to other works. See Tying Shows, Seasons, and Episodes Together for details.

Common Elements for All Works

All of the work types listed above are built on a core of common elements, extended in each case with only type-specific elements. Only a small subset of the common elements are required.

Tip: The vast majority of elements available to you in the catalog schema are optional, but providing additional metadata both helps the user to find your content more easily. If you're implementing Universal Search and Browse on Fire TV, adding this additional metadata in your catalog file will help Amazon better discover and match your content to that of other content providers for a better user search and browse experience.

The outline of a work element can be represented as:

(required) is an identifier of your choosing for the work. Each work's ID must be unique within your catalog and it should never change as long as you offer that work. The ID element is also used in associating work elements, for instance to specify a TV episode as part of a TV show and season.

(required) is, of course, the work's title. You can provide the title in multiple languages.

(required) contains the methods through which the customer can view the work: for free or through subscription. Offers can be limited by time or by region. The element must contain at least one offer but can contain as many as necessary. There are four offer types:

Offer TypeDescription
The work is free to view at any time, or optionally is free only during a given window.
The work can be watched by subscribers to the provider's service.

The outline of an offer, including optional elements, can be represented as:

(required) specifies the countries in which this offer is valid. Regions is required and must contain at least one Country.

and WindowEnd (both optional) can be used together or separately to specify the time when this offer is valid. Before and after , the offer is not shown to the user.

(optional) enables you to specify options for visual quality, audio language, and subtitles under this offer. It also allows you to define a special ID that can be used to directly launch a work with a predetermined configuration of those options.

If the availability of a given work changes, you must submit an updated catalog file with the new offer information.

The detail page for a movie, extra, or TV show displays all of the available offers and providers for that item. We display viewing options in this order (subject to change):

  • Free offers
  • Subscription content
  • Fee-based content (purchase or rental)

Common Optional Elements for All Works

So far, we've discussed the common required elements and a handful of common optional elements. Using those alone, you have the knowledge to construct a valid catalog file.

However, those elements account for only about one-third of the total available to you. All the rest of the common elements are optional and are used to provide more information about the work.

For example, there are elements for genre, certification, cast and crew, plot description, studio, images, and customer rating. For a full list, see the Catalog Data Format (CDF) Schema.

A Note About Strings

Some string data in the CDF schema, such as a work's title and description, are defined as the custom type. Localized strings allow you to provide the same content in different languages, to be used according to the user's device language setting. These strings have the required attribute (of standard type ). Here is an example:

Localized strings also have an optional attribute (of standard type ). This attribute is provided for Japanese catalog entries that specify string text in kanji. The expected sort order in Japanese is based on pronunciation (which cannot be determined from kanji) rather than characters. The attribute provides that information, typically using hiragana.

Elements Specific to the Work Type

In addition to the common elements, each work type has from one to five elements specific to that work type alone. In general, these elements have two uses: (1) to specify an original release or air date and (2) to tie works together, such as a TV episode to a TV show.

Release dates are optional but recommended, and are specified by either a (for movies, TV shows, or mini-series) or (for TV and mini-series episodes, and TV specials).

The elements that bundle shows, seasons, and episodes are required, and are discussed in more detail in the next section.

Refer to the Catalog Data Format (CDF) Schema for particulars on the type-specific elements.

Tying Shows, Seasons, and Episodes Together

TV episodes are aired during one season of a particular TV show. A mini-series is made up of individual episodes. An extra can provide a preview or behind-the-scenes information about a movie. A TV special might be related to a TV show, but be outside of that show's normal sequence.

The CDF provides elements that enable you to make those connections. When a Fire TV user browses to a TV show and sees the show organized by seasons with each episode shown sequentially in its season, that is the result of these elements.

In general, you can associate one work with another by either ID or title. An ID must match the ID of another work in your catalog. If that work is not in your catalog, you can use a title instead. The title isn't required to match anything in your catalog and is only used to group works. 

The following table shows these elements for each work type:

Rather than a title, uses a number to specify a season not in your catalog and uses a link to an external ID scheme, such as IMDb. Also, while you can choose which to use, one in each element link pair is required for , , and . The link value is optional for and , which can be standalone works.

The following illustration shows how the various elements and values are matched for a TV show when using IDs.

For instance, if a had an ID of , the values in and would also equal . Download the Catalog Data Format Examples to see fully implemented catalogs illustrating these concepts.

Requirements for Box Art Images (ImageUrl)

The element, one of the optional elements common to all works, provides the URL of an image that represents the work, sometimes called the "box art." If you don't include , we attempt to use art available from other sources such as IMDb, if we can make a match, or we might use a generic placeholder image.

Warning: Your catalog must provide valid images for at least 50% of its entries; otherwise, the integration process will fail and the entire catalog will be rejected.

Image requirements

RequirementImageDescription
TypeJPG (preferred) or PNGOther image types will not be used
Aspect Ratio16 X 9 Images in your feed should follow the 16 x 9 aspect ratio.
SizeHeight greater than px (px preferred)Images less than px in height generate a warning in the ingestion report, but those between px and px are accepted without counting toward the total number of invalid images. Images less than px in height will not be used. For optimal quality, we prefer large images (no image size is too big) that we can scale as needed.

Note: If we crop your image because of its aspect ratio, the cropped version must still meet this height requirement regardless of its original dimensions.

At a minimum, we recommend that your images meet the specifications described. Note that this refers to the box art image, not the work itself.

CDF FAQ

Q: How do I create my catalog file?
A: You create this file programmatically by exporting your metadata to an XML file that fits the CDF format.
Q: I have a TV show with seasons and episodes. What content types should I use to categorize my show?
A: Use , , and .
Q: I have a TV show with episodes that are sequential, but that do not have seasons. What content type should I use?
A: Use the and types. You will need to specify values for to represent the episode sequence.
Q: I have a TV show with sequential episodes, but that don't have a sequence number. Instead of sequence number, these episodes are ordered by their air-date. Can I still use as my type? If not, which content type should I use?
A: Use the and types. In the , link it to the using the or fields, and include the mandatory air date.
Q: I have a news-type content where the episodes do not have seasons but do have an air date. What content type should I use?
A: Use the and types. In the , link it to the using the or fields, and include the mandatory air date.
Q: I have a show with seasons, but I do not have the actual values for the seasons. What should I do?
A: You are required to include season values for a show with seasons, which prevents a bad user experience. To obtain season information, try looking at an authority catalog such as IMDb. Amazon usually recommends excluding season-based content that lacks season values, as opposed to trying to make the show fit into another content type. Omitting this content should help preserve a positive viewer experience for your users.
Q: We don't have season or episode information for our series. What should I do? Should I just make up fake values?
A: Do not provide fake numbers and episode numbers in your catalog. Instead, replace the entry type with . Continue to map the entries () to the , so that they are associated with one another through the . However, note that in , is a required field. This allows us to better match (and use other sources to identify what the Season number and Episode number are) and to properly order the episodes in the .
Q: These all seem like an awful lot of rules to remember. How can I tell what to do without having to memorize every little nuance?
A: Generally, go by what your customers would expect. For example, if you have a TV series that is a talk show that airs nightly, your customers probably will not tend to think of that series as having seasons. In this case, air date is more important. However, if you have a popular series that tends to be binge-watched by users, season and episode number will likely be much more important to that viewer base.
Q: Ok, I've created my catalog file. Now what? Can I just upload it to AWS S3?
A: Amazon strongly recommends validating the XML in your catalog file before uploading it to AWS S3. If your CDF contains any poorly formed or invalid XML, the file will be rejected by Amazon. See Step 2: Validate Your Catalog File for more details.

Next Steps

After you create your catalog file, move on to Step 2: Validate Your Catalog File.

Источник: [manicapital.com]
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