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Inhibitory Activities of Zygophyllum album: A Natural Weight-Lowering Plant on Key Enzymes in High-Fat Diet-Fed Rats.
3. Results
3.1. Total Phenolics (TPs) and Total Flavonoids (TFs)
The level of flavonoid and phenolic compounds in different extracts of Zygophyllum album is shown in Table 1. Except the hexane extract, which contained low amounts of flavonoid and polyphenol, the other extracts were found to be rich in these compounds. As shown in Table 1, the butanol extract showed the highest amount of phenolic compounds (403.46
mg GAE/g) followed by ethyl acetate (394.28mg GAE/g) and ethanol (391.65mg GAE/g) extracts.
Table 1
Flavonoid and total phenolic contents of various extracts ofZygophyllum album.
| Sample | Total flavonoid content (mg QE/g extract) | Total phenolic content (mg GAE/g extract) |
|---|---|---|
| Hexane fraction | 21.8 ± 0.11 | 78.022 ± 1.56 |
| Ethyl acetate fraction | 47.16 ± 0.23 | 394.28 ± 7.88 |
| Butanol fraction | 45.8 ± 0.23 | 403.46 ± 8.06 |
| Ethanolic extract | 40.98 ± 0.20 | 391.65 ± 7.83 |
3.2. Pancreatic Lipase Inhibitory Activity Assay In Vitro
As shown in Table 2, for the different solvent extract fractions, the IC50 values of ethanol and butanol extracts were (91.07μg/mL and 94.71μg/mL), respectively, indicating their strong inhibitory activity over other solvent extract fractions against the pancreatic lipase. It should be noted that fluvastatin showed potent inhibition of pancreatic lipase with a strong IC50 = 16.76μg/mL. The potential inhibitory effects of ethanol extract via other extracts explain the use of this extract for the in vivo study.
Table 2
In vitro pancreatic lipase inhibition assay of various extract ofZygophyllum album.
3.3. Effect of EZA on Body Weight of HFD-Fed Female Rats
By the end of the 6 weeks, the body weight in each group of rats was measured. Also, HFD-fed animals presented significant increase in body by 18% as compared to the control group. However, administration of EZA or fluvastatin to HFD-fed rats reduced significantly the body weight in comparison with the corresponding HFD group by (12 and 10%), respectively, P < 0.05 (Figure 1).
Effect of EZA on body weight of control and HFD treated rats. Values are given as mean ± SD for group of 8 animals each. Values are statistically presented as follows: *P < 0.05 significant differences compared to controls. #P < 0.05 significant differences compared to HFD rats.
3.4. Effect of EZA on Serum Leptin Level of Experiment Animals
Serum leptin level was significantly increased in HFD group by 104% as compared to control animals. However, in response to the fluvastatin and EZA, serum leptin levels in groups received high-fat diet were significantly reduced by 35 and 34%, respectively, as compared to HFD-fed rats, P < 0.05 (Figure 2).
Effect of EZA on leptin level of experimental rats. Values are given as mean ± SD for group of 8 animals each. Values are statistically presented as follows: *P < 0.05 significant differences compared to controls. #P < 0.05 significant differences compared to HFD rats.
3.5. Effect of EZA on Lipase Activity and Lipids Profile in Serum and Liver
Figure 3 indicated that the lipase activity in serum of HFD-fed animals underwent a potent increase of 65%, as compared to control animals. The rise in lipase activity stimulates lipid absorption and, consequently, led to a remarkable increase in the TC, TG, and LDL-c levels in the serum by 62, 45, and 241%, respectively, and in the liver by 127, 130, and 320%, respectively, associated with significant decrease in the HDL-c level in serum and liver by 18 and 28%, respectively, as compared to the normal female rats. However, the administration of EZA to the obese animals reverted back the lipase activity in serum by 37%, which lowered the rate of TC, TG, and LDL-c and increased HDL-c levels in serum and liver (Table 3). Moreover, fluvastatin supplementation to HFD-fed female rats was observed to improve their lipid profile.
Effect of EZA on lipase activity in serum of control and HFD rats. Values are given as mean ± SD for groups of 8 animals each. Values are statistically presented as follows: *P < 0.05 significant differences compared to controls. #P < 0.05 significant differences compared to HFD rats.
Table 3
Total cholesterol (T-Ch), LDL cholesterol (LDL-c), HDL cholesterol (HDL-c); and triglycerides (TGs) in serum and liver of HFD-fed rats treated with EZA.
3.6. Histopathological Studies of Liver in the Control and Experimental Groups of Rats
As shown in Figure 4, the section of the liver from a control rat showed a normal architecture. However, liver of HFD rat exhibited fatty cysts apparition in liver tissues, whereas, examination of liver tissue of HFD-fed rats treated with fluvastatin or EZA revealed potential protective action evidenced by reducing the development of fat cells in liver.
Histopathological studies of liver in the control and experimental groups of rats. The section of the liver from a control rat showing normal architecture; liver of HFD rats showing fatty cysts apparition in liver tissues; the liver of HFD rat treated with fluvastatin or EZA; a potential protective action was shown.
3.7. Effect of EZA on Serum ACE Activity
Figure 5 evidenced that the ACE activity in the serum of HFD-fed rats underwent a potent increase of 81% as compared to the control rats. However, the administration of the EZA or fluvastatin to the HFD rats was reverted back the activity of ACE in serum back by 36 and 34%, respectively.
Effect of EZA on serum ACE activity of experimental rats. Values are given as mean ± SD for group of 8 animals each. Values are statistically presented as follows: *P < 0.05 significant differences compared to controls. #P < 0.05 significant differences compared to HFD rats.
3.8. Effect of EZA on Serum Major Electrolytes
The findings indicated that compared to the control animals, there were significant reductions in serum Na+, Cl−, Mg2+ and Ca2+ by 7, 9, 30, and 22%, respectively, with an increase in the level of K+ by 25% (P < 0.05), observed in the serum of HFD-fed rats. However, administration of EZA to obese rats reverted back near to normal value of the studied electrolytes (Table 4).
Table 4
Serum electrolytes levels of control animals and HFD-fed rats. Values are given as mean ± SD for groups of 8 animals each.
3.9. Effect of EZA on Liver-Kidney Function in HFD-Fed Female Rats
Table 5 demonstrated that the HFD-fed rats undertook an increase in terms of the AST, ALT, ALP, CPK, T-Bili, and GGT rates by 82, 50, 123, 42, 51, and 96%, respectively, and creatinine, urea, and uric acid rates in serum by 29, 16, and 20%, respectively, when compared to control rats. Interestingly, the administration of EZA to obese rats seems to have reversed this increase back and ameliorated all indices related to liver and kidney dysfunction induced by high fat-diet.
Table 5
Liver profile indices (AST, ALT, ALP, GGT, CPK, and T-Bili) and kidney parameters (creatinine, urea, and uric acid) of control and experimental groups of rats. Values are given as mean ± SD for groups of 8 animals each.
| Control | HFD | HFD + fluv. | HFD + EZA |
|---|---|---|---|---|
You may need a serial number and product key to activate some Autodesk software.
Note: If you are on subscription, you may no longer need to enter a serial number or product key; you just need to sign in. Serial numbers are never required for launching products with network licenses.
Serial Number Locations
Serial numbers are unique codes associated with your Autodesk Account and a particular product that you have purchased or is otherwise available to you. For 2014 versions and later, you can find them in Autodesk Account, on the Management tab. Depending on your license type, you may not need a serial number to launch your product.
Autodesk Account
The Management tab of your Autodesk Account page stores the serial numbers and product keys for each licensed product.
Note about serial number visibility in Autodesk Account: Only account administrators, such as Contract Managers and Software Coordinators, and Named Users with assigned software benefits will see serial numbers in Autodesk Account. You are the account administrator if you purchased a software subscription using your Autodesk Account or were assigned the role of Contract Manager or Software Coordinator by your company. If you do not see the software you wish to activate in your Autodesk account or see the message "Contact your admin for serial numbers," you need to contact the account administrator. Only an administrator can assign you as a Named User or End User and give you permissions to download and activate the software.
Education Community
When students, educators, and educational institutions download products from the Education Community, the product serial numbers are found in the following locations:
- Displayed at the time of download
- Sent by email
- On the Management tab in Autodesk Account
See:Find Serial Numbers & Product Keys for Educational Licenses
Physical Media
Serial Numbers do not appear on software packaging for Autodesk software versions 2014 and newer. Serial numbers for these versions are emailed to you after you place an order or can be found in Autodesk Account.
If you have physical media (a DVD or USB key) for a 2013 or earlier product, your serial number and product key will be printed on the label of the product packaging.
Example of label from 2011 version. Format is similar for 2010 - 2013 product releases.
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Product Key Locations
Product keys are codes that identify each product version.
You can find product keys for recent versions of your product in Autodesk Account, on the Management tab.
To look up a product key for a particular product version (2010 and later), you can also go to: Look Up Product Keys.
Older product keys
If you can't find the product key for older versions of Autodesk software (2013 and later), you may be able to find it in a text file in the installation folder.
To determine if your product key is available in your installation folder:
- Using your installation media, (USB key, DVD, download folder, etc.) navigate to the folder that contains the setup.exe file for your Autodesk product.
- In that folder, look for a file named MID.txt, MID01.txt, MID02.txt or some variation on that name.
- Open this file in Notepad and verify that the product name is correct.
- The first five characters of the part number should also be the product key for that product.
Example:
MID: Autodesk_Design_Suite_Ultimate_2012_64bit_SWL_ENU_C009_EXE1
Product Name: Autodesk Design Suite Ultimate 2012
Platform: Windows XP/Vista/Win7
Media: ESD
Part Number: 769D1-05Y001-P503E, 769D1-05Y001-P504E
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