ACID Style DJ 3.0a serial key or number

ACID Style DJ 3.0a serial key or number

ACID Style DJ 3.0a serial key or number

ACID Style DJ 3.0a serial key or number

@kumpulan Serial Number

Transcript

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name:Welcome to manicapital.com sn:ABDPG-AANSY-AVNJO-MKJVA-HJUNA Windows ME H3QQR-HXVTY-TR4XJ-YQV3Y-KVPTG Pinnacle Instant Copy v Retail name:Welcome to manicapital.com sn:AAOUQ-AAHLX-GXPHH-GANRC-VNYXA Photo Brush sn:BBJN-PO1T-SSZR unlockCF-4FAF Pinnacle Hollywood Fx V SN: HFPROM AutoCad Serial: CD-Key: 5X8NUG Photo2VCD Standard v Name : TEAM CAT Serial : 0AC7A1F4B Name : Ulysses Gaze Serial : 00C75FE66 NORTON 1C6EF-DDA78D-ABFDFE-3BF8F15E VTRXTBCFXCR6JH8DBF92 photoshop SC2 Acd see v 4QSD7HDGSJ-DRXQDJ7-D76 4QSD7HDGYL-4GT-D76 Adobe Photoshop CS ME winamp name:??????¼?????_??????_manicapital.com Code:2V9WTMBWTXF7W Nokia Multimedia Converter loginatriot55 snuiQL5Nv5lg59+IlsF7eqwG4lRA= rm to mp3 serial Name: Registered Serial: YP8-BKMNDH-A42NG3-AH5TFZJ23CPFDK-U07VC7-NX1J0M TMPGEnc DVD Author v Code: TDNVUA6R-F84Y-4YR6 ACDsee standard ACDSee PowerPack COREL DRAW 11 ISO Serial: DS11CRFYCK DS11WRCVAH manicapital.comL ### Begin License ### PD:{HyperSnap-DX 5} LO:{Matthew Villamaino} SN:{e} NC:{1} LD:{} KI:{HPHS-0} FS:{1} KD:{ EK:} ### End License ### DVD X Copy Platinum Password: User Code 1: User Code 2: License ID: Activation Code: Registrar por Tlf. note: License: Password: Nero Burning ROM vB Enterprise Edition Nero manicapital.com [Unlimited S/N] s/n: 1A s/n:1A s/n:1A s/n:1A Nero Burning Rom V SN: 1A SN: 1A SN: 1A SN: 1A SN: 1A SN: 1A Nero Serials 1A 1A 1A 1A 1A A5DEF40BdgCEBBC44E0 9BE15F30FA4 Ahead Nero Ulta Edition 1A 1A 1A 1A 1A WinDVD Platinum v eMail:[email&#;protected] Password:crsky Key:VV3QY3UD-FSB6PHVK3KRZKYY7VJ8SJJ2 Card Power DvD xP SERIAL : DX Nero 1A 1A 1A FA45CAB Jet Audio xP SERIAL : JADWB-FB3BC78ADEC Download Accelerator Plus TPH Music Match SERIAL : UK-GLJQK-4LJJ2KL37K manicapital.comL fgctslijnunpp64 1 fgcmzv×lgokri1 11 Real Player SERIAL : PowerDVD V AK MVG Customizer Xp V SERIAL : ph PowerDVD V DV WinBoost SERIAL : 8Q7F8-WXUD4V Premiere 6 MBFB Sonic Foundry DVD Architect v Computer ID : 1PYNKQ0 Serial : HPFKXPD17S-5P5EBR Sound Forge 6 ID :: 01E39LL Serial :: HK-2VVYMNRVYE-SB32JP Super DVD Ripper V CAADC7FADAF9BAA5B8E 2CD5DDE4DFF8CC2 Screen Saver Builder SERIA : 5C7E5BAC0CFCC Window Blinds SERIAL : Name : James D Goldman : S.N : WB f 74Cf WindowFX Enhanced SERIAL : Name : Ghost : S.n : WXGAGE WinBrush SERIAL : User name: TEAM LAXiTY : Serial: 2A4Z5-WDMEAP Cool 3d v SERIAL : MLVC DiscJuggler SERIAL : CDJDFTU Nero Burniy Rom SERIAL : Clohe CD SERIAL : Name : ECloZion FB89FFB CD Ripper SERIAL : Name : manicapital.com : S.N : MP3 CD Maker SERIAL : Name : Michael : S.N : Copernic Summarizer SERIAL : SU -Macromedia FreeHand 10 Serial Number : FHW begin_of_the_skype_highlighting end_of_the_skype_highlighting -Macromedia Generator Serial Number : FGW Get Right SERIAL : ———————————————————— ————-Macromedia Dreamweaver Ultradev Serial Number : -UDW -Macromedia Firework Serial Number : -FWW -Macromedia Authorware Serial Number : APW -Macromedia Director Serial Number : WDW Macromedia Director Serial Number : WDW -Macromedia Dreamweaver Serial Number : DWW -Macromedia Extreme 3D Serial Number : WDW -Macromedia Firework Serial Number : FWW -Macromedia Flash Serial Number : FLW -Macromedia Fontographer Serial Number : -Macromedia FreeHand Serial Number : FHW -Macromedia Generator Authoring Templates Serial Number : FGW -Macromedia Xres Serial Number : REW AND MORE MICROMEDIA SERIALS: Authorware 5 : APW Dreamweaver 1 : DWW Dreamweaver 2 : DWW Dreamweaver 3 : DWW Dreamweaver 4 : DWW Dreamweaver UltraDev 1 : UDW Dreamweaver UltraDev 4 : UDW Drumbeat (ASP) : BDA Drumbeat (JSP) : BDJ Drumbeat eCom : BDE Fireworks 1 : FWW Fireworks 2 : FWW Fireworks 3 : FWW Fireworks 4 : FWW Flash 3 : FLW Flash 4 : FLW Flash 5 : FLW Freehand 8 : FHW Freehand 9 : FHW Freehand 10 : FHW begin_of_the_skype_highlighting end_of_the_skype_highlighting Adobe Photoshop Serial : Fast Browser : Name : Feras Baraviy Serial : Ravisent CinePlayer DVD Serial : FIRNV Company : Aseel Computer Center Serial : 2AXJPM-AGUUW Ahead NiroMix Serial : Setup Factory Serial : SF Aqua 3D Screensaver Serial : CABE-7D9FA-5FE4 ScreenLock Professional Code / BCD1 SWF-Browser Name : Gecco [odt] Code : CFFD AWicons Name : Feras Baraviy Serial : True Screen Saver Builder Reg ID : MNCHK4J03EVKE5O0OFC52IHJU3O9JZKP Reg Key : FD3 Be Faster Name : Feras Baraviy Serial : Talisman Name : TNT!krackers Serial : CD Ripper Name : manicapital.com Serial : Vizros Plug-ins Registration Code: TNM CompuPic Name : Feras Baraviy Phone : Serial : 1BB74BZH4Q Win ACE Name : Feras Baraviy Serial : ACEQTV2TRT14RHFG64TUIDZKTG4 Cursor XP Plus Name : Frank Wheelock Serial: O6IH7-M31JS-SNHISP7J Winboost Name : Feras Baraviy Serail : AT9D3-DLXL2U WinVCR Plus Serial : WCP-DRR Customizer XP Serial : ph E-Icons : Name : Feras Baraviy Serial : CORL Final Data NT Serial: NTE Internet Timer Name : Liquid8 Serial : LDFEAVSAFCW Kai Power Tools Effects Serial : TF70CRDJVR Jaws PDF Creator Installation Password: UmeshMacarthur Serial: HFF0DETFIV2LNE0C Pretty Good Solitaire Name : 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Pro MSF2K-PBPHUHU Advanced Office XP Password Rec. Pro AOXPPR-PRO-TADFHHTHAF Advanced Paradox Password Recovery MSPPDWMRA Advanced PDF Password Recovery PDFPEYSVY Advanced PDF Password Recovery Pro s/n: PDFP-V8FW7J-HDUY-NXNFA-Z6V3CFXXE AlamDV Name: KHS Inc. s/n: dqonbaobazyxwvut AlamDV name: Claudio Duffini s/n: bspdopxmaziansxm Alarm Master manicapital.com Code Alcohol % v s/n: AL12PPPPAFAE4rneMMz Alive MP3 WAV Converter manicapital.com CodeB66E-E67E6 Analityk System Wspomagania Dec. PL wAo6xQ-ontcA8-QpGEii-v24iSE Animated Screen manicapital.com Code:E3BBBC00 Animation Masters Anim-FX erptr87 Anti-Hacker Expert v [email&#;protected] Code:2B36F-FBEA6-F66FB2FE Anti-Trojan Name:brother-warez Serial:5ABAACCC AntiVirenKit 12 Professional PL trial 26BFFCCF1D6D56EAF8E0EE2F Applet Button Factory Name: 12aew s/n: 9j8f5 Aqua 3D screensever Name: DARRYL WILSON s/n: FLDP-MXMS5MBFBV3T-JBTT Aquatica Waterworlds manicapital.com Name: Wanlop L. s/n: TMOPZ-KWVZA-SEIQO-JYGXA Architecturals for IntelliCAD Name: Team PARADOX Company: Team PARADOX s/n: ZV6W-DDN4-RX3M-YGG6-N36A-DHZB Ashampoo AudioCD MP3 Studio Name:manicapital.com Code:CSN77B5AA0B54N AtomixMP3 v 9KP-PMFC Audio CD Copier Code Audio Record Wizard manicapital.com CodeCDC-9FD Audio Record Wizard Name: Megido Group SN: AFBCED SN: EBA3-A73F-DF5A SN: 1BAB8AB8 Aurora v name: Lunatic s/n: AutoCad PL Serial: CD-key: 5×8NUG AutoCad i s/n CDkey 94ED61 AutoCad LT PL s/n: CDkey: T4ED6P AutoCad s/n: CD Key: DXW9L4 AutoCad Mechanical Desktop VVWVAM VVWVAM VVWVAM Autodesk 3D Studio VIZ R3 serial : cd-key : SWP9UV Authorize : Autodesk Actrix Technical name: huh organization: dsg s/n: Autodesk Architectural Desktop R2 serial: cdkey: 5X8NUG Autodesk Architectural Desktop 2i Serial: cd-key: T4ED6P Autodesk Architectural Desktop serial # : cd-key : T4ED6P Autodesk AutoCad serial: cdkey: 5X8NUG Autodesk AutoCad Educational serial: cdkey: GX8NUG auth: FFD54A5A Autodesk AutoCad Serial #: cd-key : T4ED6P Autodesk AutoCad Data Exchange i Autodesk AutoCAD Land Development Desktop Release 2i s/n: cd-key: T4ED6P Autodesk AutoCAD LT serial: cdkey: 2ZC3DJ Autodesk AutoCAD LT i serial: cd-key: T4ED6P Autodesk AutoCAD LT s/n : cd-key : T4ED6P Autodesk AutoCAD Map i serial: cd-key: T4ED6P Autodesk CAD Overlay s/n: cd-key: 2ZC3DJ AuthCode: 08BB Autodesk CAD Overlay i serial: cd-key: T4ED6P Autodesk CAD Overlay serial # : cd-key : T4ED6P Autodesk Civil Design Release 2i serial: cd-key: T4ED6P Auth : no requiere Autodesk Civil Design serial # : cd-key : T4ED6P Autodesk Land Development Desktop Rel. 2 Serial #: CD-KEY : T4ED6P Auth : no requiere Autodesk Land Desktop serial # : cd-key : T4ED6P Autodesk LightScape name: EzD company: PWA s/n: code: 6AAF9 Autodesk Mechanical Desktop 5 serial: cdkey: T4ED6P Autodesk Mechanical Desktop cdkey : auth key : TLSQBS Autodesk QuickCAD Millenium Edition R7 GENESIS First Box: Third Box: Autodesk Raster Design 3 s/n: key VZY8QQ Autodesk Survey Release 2i serial: cd-key: T4ED6P Autodesk Vip Library 4Q99 Actrix Business Serial: Autodesk VIZ 4 serial no. registration no. Autodesk Volo View Auto Odtwarzacz manicapital.com Numer: Numer: Poland’98 Haslo: Auto Odtwarzacz / Auto Player v Uzytkownik / User : shadowRUNNER Numer / Number : Poland’98 Haslo / Password : AutoPlay Media Studio Retail s/n: AMS key: C-B2CEA Autoplay Media Studio s/n:: AMS Key: E69ABBC Autoplay Menu Studio s/n: AMS Key: C-B2CEA Avg Antivirus Pro 70ZJ-TV5IPE-CY2SX-ZKF-TZ4DD9 Avi/mpeg/rm Joiner emz4ud AVI/MPEG/RM/WMV Joiner manicapital.com Code:VFJE2OFKK AzGuard Name:MAGNUM KeyBDADBFAE-CDFED9 s/n: Big Scale Racing EDA66 BiznesPlan s/n: BPD BlindWrite Suite s/n: 5LFG - ENPV - EI5W - 5LFJ Code: YRB1F1J1YDYU7MEJ BlindWrite Suite Code:5LFG-ENPV-EI5W5LFJ BlindWrite Suite Code:5LFG-ENPV-EI5W5LFJ BlindWrite Suite YRB1F1J1YDYU7MEJ BlindWrite Suite name: bhljkrth77vkvk s/n: 9TEOQ0TD6O16DTEJ BootXP RC1 Name: Megido Group SN: BXP Borland C++ Developers Kit BDSWW Borland C++ Builder 4 s/n: key: xcx×0 Borland C++ Builder s/n: key: 4eb go back and enter 4eb Borland Delphi C/S s/n: 8exfx0 Borland Delphi Enterprise serial: z9j8pum4n-c6gzq key: rwjw Borland JBuilder Pro s/n: key: c Borland JBuilder Pro s/n: xa?2xtt-rpvb9 key: 5p7-?rz Borland JBuilder 4 serial: xa?hrsubgs key f2jg BBack4WinXP Name:[email&#;protected] Code:GE Backup DVD s/n: ASRO2-IFHT1-AWNA9TRE89 s/n: FIATERA3-SQFANW71 Backup To CD-RW s\n: Banner Maker Pro Name : MackOi DBC Serial : Banner Maker Pro Serial: Bartez Movie Library name: AAOCG tEAM! e-mail: Advanced Art of kracking Group code: Befaster x name: Mister Stop CCakewalk Music Creator CWMC Cakewalk Pro Audio s/n: cwpa key: Caligari Truespace s/n: Camedia Master s/n: zcmemk Camtasia Studio Name:Ghostbuster SnDEC8E Catz, Your Computer Pet s/n: CD Autorun creator CD-Bremse user:hacekw serialEE51FCBDB6 CD/DVD Diagnostic retail Name:manicapital.com Company:manicapital.com SN: L CD/DVD Diagnostic multilingual s/n: L CD Mate Deluxe name: TEAM CAT s/n: CMAA0EATAA CD Mate Deluxe Name: Oxide Owns Company: Oxide s/n: CMNC8DOCF7 CD Mate Deluxe Name: TEAM CAT s/n: CMAA0EATAA CDRipper Code CDRLabel 6 Line1: Line2: CDRoller Name: NeRo s/n: CDRoller name: NeRo s/n: Cdrwin c Your Name: Mark Brown Company/Email: Power Shells Unlock Key: AEFEC Check Key: 9E56C7CDCE7 Cdrwin 50R0R-CDR CDSpace cd-key: SF C-GEO LT s/n: Chameleon Clock Code: PW-LOCK-6YJALU3T-PN2ZK Check Favorites Name: Megido Group Company 16B66DHTL51PX9NPY 0DBX0L1US-1CTEMI7 CheckIt Suite TC Chem Office Ultra serial: keycode: FRKNLGMPT Cinema 4D s/n: A8C4 Cinema 4D Demo A8C4 Clasp name: Chafe/TEX99 s/n: EX CleanCenter manicapital.com Code:EC0 CloneCD name: Randal Dudley Dudley s/n: CloneCD beta 30 CloneCD CloneCD CloneCD name: JUSTiC s/n: CloneCD Collins (slownik PL-ANG) ZA YA Collins (slownik PL-ANG) YA Color Pilot SN: SN: SN: Contractus ef-8d5c Cool Edit v Name: MFD Corp. s/n: Q17EXF5U Cool Edit Pro Name: Peter Quistgard s/n: YQOQ7L CoolIE Name: TEAM LAXiTY s/n: vqvOfKTyvmnuTiDibSXG Copernic Agent Professional s/n: CC s/n: CC CopyDVD Username: dbrain e-mail: [email&#;protected] regcode: 6M6RBCTSKKHYH6EDBUAO Copy DVD to CD-R s/n: 6MRBCTSKKHYH6EDBUAO CopyToDVD SN: D1ONKDBPBBAP5SEJ SN: I1TOKLT33AEKASEJ SN: QDX00R7PWNFAUQEJ CopyToDVD CodePURVRID9V9LREJ CopyToDVD Code:H87IFRSPUNVVYMEJ Corel Bryce BF50CRDWDB Corel Custom Photo SE ccp-psfownz Corel Draw DR9NRW Corel Draw 10 English D10NRT76 Corel Draw Graphics Suite 11 DR11CRDDGW Corel Graphics Suite 11 DR11CRDDGW Corel KnockOut Corel Painter 7 PF70CRDSZH Corel Painter 8 pf08cumdcn Corel Print Office P02NR-F Corel WordPerfect Office WpPHX Corel WordPerfect Office Professional PWQ Corel Xara coverXP Pro Name:aura/bf! 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Serial License key Finale DVDA FinePrint Enterprise Code:FK6V-Q9URMZZZ FinePrint PDF Factory Pro Code:EEDCWP6Y-BQNZ Code:EE6L-B3HJ-RP6Z Code:EEZS-5VH2-RTH2 FineReader PL s/n: C5A0 FireBurner manicapital.com Code Flash Catcher Name : manicapital.com Code:1ChBgdViNt FlashGet (JetCar) v PL FLASHGET EC2M7 Flash Hunter Name: ReanimatoR^LasH Code: jwPIdnpcr8vTixmyeH1j FL Studio name: ChristianKolberg password: Fonty Pro PL TPP66C Form Pilot Home Name: Bean Organization Bean Code: Formz RenderZone 3.x s/n Z code IQBGKS3U57APME9G FotoOffice PL 4AF5FBFCE3FAE36A31 Français Translator s/n: 6CABFCAE7F Freehand FHW Frontline Attack FUBP-EYNZ-KFXC-SKM2 PNN6-G2N7-XR3B-AHT8 FullDisk Code:5FFDD43EFCE4F6 FIFA 7UFACXH-ANE4-LDEV GGalleon 3D Screensaver Registration Name (case sensitive): RICHARD K. GROSS GetRight GetRight e GetRight beta ELAMXX-LADND-2EYP1EPPiC-6ST2A GetRight beta 4 GR50EM-W6ZRG-Z8WNP7KSZE2LW GetRight beta 5 GR50EM-W6ZRG-Z8WNP7KSZE2LW GetRight MJVLW8-M92PG-TGMVMB0NWA-OS43P JSM93K-9S59H-V7TJG95Q-OV2W1 TAF9PO-INDHJ-TPSFDIMI-U7MA9 NOGLVC4WA-1BDIN-3M0A3-HQEUB BG2SP5-UG4TD-6J63K-UHAEHWAM GetRight final ALLDRE-PAGDM-LE3PWPEPSMT8A GetRight s/n: NTSXTE-CB1F2-L6H9i47oKK-FGK6Q GetRight a UJPROR-IDVQJ-OGPJP-SUBU9WCAB GhostView THG GoldWave Name: REANIMATOR Company: LASH s/n: QHGKVQB Guitar Pro 4 DEMO User ID: AROB Key ID4: Guitar Pro Multilingual User ID: AROB Key ID4: HHard Drive Mechanic s/n: 65B Hare Name: BaND ENFUSiA s/n: Hollywod fx pro for pinacle studio 7 serial : HFX4V-STRR Maschine: ID HomeSite s/n: HSW HotDog Professional Install ID: Q9SC5PEJ12P0S3T s/n: D41D50BD85B HyperCam v Name: Gordon s/n: KLWO-XEBS-PPKL-LGZA-TSVI- DZOA HyperCam v Name: Dynamite/KNO s/n: KLWO-XEBB-EGPI-AULX-NIWZ- YLMN IIE++ Regcode: E7DD ImTOO DVD Ripper manicapital.com Serial CodeF-EE3CEA2 IncrediMail Xe Infax TOA3C6D3G6N3B6F5 Infini 4D Installshield ADPROTX14IS IntelliCAD D4NZ-A3DZ-RKFH-B46N6NHD-AZWV Internet Download Accelerator email:[email&#;protected] Nameverdays Code:jb5cG5reY4oQU Internet Download Manager Name: Team ElilA s/n: $ED93 Internet Translator 7FA6-D0ECE 42EBCC Internet Translator 62DEBD-CD99 IPScanner Nameverdays Codebd-d24b31a2-bbba9a irFanview Name: Tested by SoftReef s/n: Irfan View32 v Name:CZY Code iZotope Ozone Winamp Plugin Name: San Perese Email: [email&#;protected] s/n: iZotope Ozone for Winamp retail Name: Nitrogen[TSRh] Email: [email&#;protected] s/n: JJPEG Format PlugIn for IrfanView s/n: IV s/n: IV s/n: IV s/n: IV Jpeg Optimizer 3.x AY KKai’S Photosoap EY Kai’s Power Show KPSM1 Kai’s Power Tools TF60WRDUHV Kai’s Power Tools Vector Effects VF15WRZDHC Kai’s Super Goo Kai’s Super Goo ez KaMat McKiler/krackPL’99 GD Kody Pocztowe Name: PLASTEK Serial: BCVWQLWCNGMDESDG SysID: Komputer Autor: format_c Serial: i Kulturystyka i Fitness MAFYBPUMV54N Kurs Komputerowy Flash PL Kurs Komputerowy HTML PL LLangenscheidt YDP YF Language Localizator s/n: B1FE Leksykania - Angielski AA84 Leksykonia - Niemiecki - Bazy ADTVZ Licznik Polaczen Modemowych 3coin Lock Files & Folders XP v Name: TEAM ViRiLiTY s/n: erq MMacromedia Authorware Attain APW Macromedia Authorware name: Cats Are Cool s/n: APW Macromedia Contribute CTW Macromedia Director WDW Macromedia Director WDW Macromedia Director WDW Macromedia Director MX Name/Company: Java2k sn: DRW Macromedia Dreamweaver DWW Macromedia Dreamweaver Ultradev UDW Macromedia Fireworks 3 FWW Macromedia Flash FLW Macromedia Flash FLW Macromedia Flash MX FLW FLW Macromedia Flash MX Final FLW Macromedia Freehand FHW Macromedia Freehand FHW Macromedia Generator FGW Macromedia Studio MX WSW WSW Maddog IPZ4XY MagicTweak manicapital.com Code:Z5CK22JZ Magic Utilities v Name: FreeUser Code: DD9DGDGD Magix Music Maker 7 UK-GLJQK-4LJJ2KL37K Mapa Europy ME Mapinfo Professional PN: s/n: S Marine Aquarium Time ATIME2ECJYGKODZ7 Marine Aquarium s/n: KILLA23LX2WCVO4 Matematyk _________________________________ KUMPULAN SERIAL NUMBER(SN), REGISTRASI, CRACK, KEYGEN SOFTWARE FastStone Capture Company : Agus Studio Serial : OPOMR-IJDIU-BPVSR-KAFJI ——————————— Tune Up Utilities Name : Everybody Company : Agus Studio Serial : F1UTW6UQ-3AXPDDB-PVHHR8MA7U ———————————– ACDsee 10 Photo Manager Name : Abu Afif Serial : DFNDVHDJ-3PM35V-4QQM28C ————————————Desktop Maestro Name : Heiko Koese Serial : 4EB76A-8FEB2C-D41FB9CC4FCE ————————————– Registry Mechanic 7 Name : Abu Afif Serial : BFEF9FD-DCA7CAA-7AEF ————————————— Axialis Icon Workshop Name : Abu Afif Company : Agus Studio Serial : —————————————UltraIso build Name : Agus Studio Serial : 57CBBBB4B ————————————– Nero Ultra Edition Serial : 1KMK6C —————————————– Acronis True Image Home 11 Serial : X72WS6RV-ZNWEC-PLPQL-Y2GN7 ———————————————Power Iso Name : TEAM EXPLOSiON Serial : GLZYYYI-IE5U8-EWFKX-3IQYZ ————————————– Your Uninstaller Name : Agus Studio Registration Code : FXGGD-Z2FURNQKNXEC-N0X3GC-8GHKAF-TUWWP6-VGB25K0T1G5M-CYTT0F ———————————— ACDSee v (C) ACD System —————————– Page Maker 5 for Win —————————– Power Quest Partition Magic pmeneu ——————————– WinBoost GOLD Edition WB Registration Code User Name: TheBrabo 5T5K4-DVZ7A-AC4T —————————— WindowBlinds Enhanced Name : Team BROKEN Serial : WB-cagd ——————————Delphi Serial #: z9j8-pum4n-c6gzq [ Key #: rwjw ] —————————— Linguist License Agreement ——————————— Adobe Pagemaker 03WR ———————————PhotoShop PWWR ————————————Adobe Photoshop ————————————Macromedia Director SN:WDW ———————————— Macromedia Flash v FLW —————————— Macromedia Freehand 10 FHW begin_of_the_skype_highlighting end_of_the_skype_highlighting —————————Corel Draw 9 Serial#: [Key #:fexx0] DR ———————— rekso translator Serial Number : RT Password : ar ——————————System Mechanic Pro vf Unser ID : ^XmoenseN^ Serial Number : P ———————————manicapital.com: 03ACABAA0 ———————— Active ScreenSaver Builder Name : ^XmoenseN^ SN : 03EDBCA92 ———————— AquaReal AREFS3D0AJ-CE1J ———————— Need for Speed – Most Wanted SN :P LNKML-S49X-DR7W-6MY9 ———————— Condition Zero SN : 58V2E-CCKCJ-B8VSE-MEW9Y-ACB2K ———————— WarCraft III SN : MNZBKJ-HIP9-R26ODP-GL3KCR8W ———————— Partition Magic SN: PMFR ———————— Visual Basic 6 CD key : ———————— ACDsee 6 PowerPack SN : ———————— ACD Pro SN : DKXDVHT-3V ———————— Abby Fine Reader 9 FPXF FPXF —————- ACDSee Pro D2BDVHTMKHY-3JQ5RV8 D5WDVHT-3YBSYQ-K7WK —————Ace Utilities Pauline Clark 0QGDC11BMJ9W1-AT1V4N-8RHMB3 —————Adobe Photoshop CS3 Mac OSX —————Advanced System Optimizer David Louis 0Z15YH-C41M2U-T7GVVG-YCA0PB-6NP01H —————Agnitum Outpost Security Suite PRO ——— OUTPOST KEY BEGIN ———0xmXh3psv8QjXan2kClW5OjUH9M3cPs /rIzHY /bmjt/A/0r4OjrGvE1ljeTitsvGoUrQYP3b4SyfJ2 V kBxMA6bOnxWpLKOhV4ZQRlUI6dZ0uldH3KO 7yEDPcL qhG0KFDmTUOqZ+3wV0OacgAaDeQSrEewAIc KMi3uhT 32UeFQqV4q/fjSLu86r3yX/gXdWdGRayYXw== ———- OUTPOST KEY END ———– —————AMS Photo Effects AT4RE HK4SAUTRE34R5T5REGHTFIRGTFR8ET4 TEAM DVT FFDFDJFFJDFJD35DHR3FJ39FDFD8FH38F —————Apex Video Converter Super any name (sembarang) FBA19A51EC11AF99BFA 4AEC6 9A50DB6B3D98BD48C86FFE35AB 0BAFF00D 3CA5BCD3E1FEC23DCD3 27E46CA6D CBBB0BADBA6AFF2FFCDA CAF54B7F1 A27CBBEFAAEA8DEFF0EE1 A1A1CAB2B CCEF4FA31C6DD08FA6 8C5EAE90B6E2 C35BBD9CE5CB 8AB89B ADBEEEDA09F0E46DCAF6F5A89 6C8F1FDF19C0 60BFAABF0E4E1BDBBA43 0EA C6ABDAA15EAB5DDBCB0C3 2D4C7DACC65CA —————ASHAMPOO AUDIO STUDIO AMS3AAY4D6-IYKD5P —————Ashampoo music Studio Sam Lenard AMS3AUESFQM Foad Filt AMS3AKTFC99G —————Auto FX Software Mystical Lighting M —————Babylon v6 Che Guevara QZDSEBF94P3K5AUVS8NC6T Rochelle Kessler X9TKT2UWV66SZ4W2R8WKMPT —————Babylon Pro v 1. uninstall previous version then delete babylon folder. 2. reboot manicapital.com installation use this User Name: ieccorp License Code: 9MGGMD72WVV3RFJER94XWVHYT manicapital.com the patch supplied et voila!!! Heiko Koese 4EB76A-8FEB2C-D41FB9-CC4FCE —————Disk Washer ABBCDDISKW —————manicapital.comv [email&#;protected]_PiraTiX 0HEH9M-T6R7CP-FX5RAB-N49FP6-WN8BQP7JD1BX-FU6 —————EinZip v Whi5tleR ALN8V-QFLFD-TEFXQDNDV8N Punks Not Dead JFCXA-GZ8U7-MLAKC-RQ3UNXXKK7 James Bond NUD6U-GQMLEJJ6TE-V0UNK-J49FK MaNdRaKe 5FK7Y-VLAVUE-V2RG8-HNZCY-P4N1L —————EVEREST Ultimate Edition v HJ8ZOJ-HUX-0AA9RF-XFTD IRX8HP4L47S54SZ74TPFTJ8ZN —————microsoft microsoft office xp exel xp S/N: P-JW32P-6HHGWQ8Q-FV2TC ———————— EA Battelfield S/N: DYTB-8YKDKFSVMXT ———————— Microsoft Microsoft Office Visio Professional S/N: HCFPT-K86VV-DCKHCCR-FM6HW ———————— Nero nero 7 S/N: CN ———————— McFunSoft Inc Video Convert Split Merge Studio Build S/N: Name : Sezgin Mutlu Organization: Turkey Serial : VCSMS ———————— Ahead Nero S/N: 1CE5MA2XCA-K29M ———————— Adobe Macromedia DreamWeaver MX S/N: DWW ———————— Adobe Macromedia Fireworks MX S/N: FWW ———————— Great Editing program…Macromedia’s version of Photoshop NetObjects Net Objects Fusion 10 S/N: NFGR ———————— Hooalsd Kinoma S/N: ———————— Valts Silaputnins Proxy Switcher Pro Build S/N: Name: Sezgin Mutlu Code:VMTVAXHQ-UNPHO-AN11Y-NP2XI ———————— Brasfoot Brasfoot Name: Paulo Barbosa da Silva Junior S/N: ———————— Avenue Productions GmbH Web Jewel FX, Version S/N: Maschinen-Code: VPRNGEMETCQKCNM ———————— Passender Freischalt-Code: WVBNAPWXLJNEHYSUDXOG ———————— Microsoft(R) MS Office Project Professional S/N: BHFYK-9RTKRRV3J6-XJ-XQ3Q8 ———————— EA Need for Speed Carbon S/N: DPQYKFG5-GBQTQQGD | TWSALZVMKQS2-F9EL | QEFP-MA2W-MNL8-DKES-KY2C | 3WBB-HGFK-4CCH-C5MD-XEXW ———————— PC Tools Registry Mechanic Name: Kim Weems S/N: Kim Weems 62EBBE-CCAFE-8BC7-CD-EA8C ———————— Microsoft microsoft word S/N: CN ———————— Rony manicapital.com S/N: DXNHF-B6XT7-P7BVGMBJ-GUGXE-G3E9A ———————— Ashampoo Burning Studio 6 Ashampoo Burning Studio 6 6 S/N: BRS6APA5D4C or BRS or BRS6CDEEBAAE92 or BRSACCB or BRS67ED-EEDFD8 ———————— THQ STalker S/N: FKS4JTQ-GZTM ———————— Microsoft Microsoft Office enterprise german S/N: KGFVYB-8WCK9KTGBC7D8 ———————— Firetrust Mailwasher Pro S/N: MTI4Oj9Sw9ZJQ2nY0BppZ4XYnfq6lI+APqSvqvj +AC1H0HEY5CJJZm7ESlpMsEXM0If2SyqCFsW R4W8w2rK0nrBgIWVAXAPo1xmV+BjF1VdZYnf rklm6hlvJLHqkVW2ilEFdEQA9xeD9ovQAR 0SXyZxoFFcpnZMF9m8oJc7mOlURVOzk6c3Ry dWN0dmVyOzE6MTs2Om8tc2FsdDs4OjI4ZDU 1YWRkOzQ6bmFtZTsxNTpQYXVsIEVzY2hiYWN ———————— Firetrust Mailwasher S/N: MTI4Oj9Sw9ZJQ2nY0BppZ4XYnfq6lI+APqSvqvj +AC1H0HEY5CJJZm7ESlpMsEXM0If2SyqCFsW R4W8w2rK0nrBgIWVAXAPo1xmV+BjF1VdZYnf rklm6hlvJLHqkVW2ilEFdEQA9xeD9ovQAR 0SXyZxoFFcpnZMF9m8oJc7mOlURVOzk6c3Ry dWN0dmVyOzE6MTs2Om8tc2FsdDs4OjI4ZDU 1YWRkOzQ6bmFtZTsxNTpQYXVsIEVzY2hiYWN ———————— Grisoft AVG Internet Security Serial Feb S/N: 75I-TH1RPCS2AL7GDPRFV ———————— Uploaded Name:gisoft !! Real name is Grisoft, very sorry for wrong typing Gubben Grisoft AVG Internet Security Serial Feb Name: Internet Security Serial Feb S/N: 75I-TH1RPCS2AL7G-DPRFV ———————— UniBlue SpeedUpMy S/N: 00XHEHM5TY2P-QEZJWB-V8UUHN ———————— manicapital.com ns/ppc/sp/gog/?engine=adwords!&keyw ord=%28speedupmypc%29&match_type=&gc lid=CI75m7fqmIsCFRPdPgodSQuIDQ Roxio Toast Titanium S/N: Y7LN-B90URV ———————— Ahead Nero S/N: 1CE5MAAXKM22 ———————— Cyberlink Power DVD 7 S/N: RQRMN-CS8GTAHKQX-Q5QW4-NQMWE-H2RLU ———————— TuneUp TuneUp S/N: Name: smmovi Corparation/Firma: smmovi FE6NK-1WGDBY9L-G6EUP-TRKESSXU ———————— THCQ Supreme Commander S/N: SR9HWXZb-DR6X-C4GS-X1VB ———————— On the GO Soft Passport Photo _nt_5 Name: manicapital.com htm S/N: PB2fe5c ———————— EA Command And Conquer 3 Tiberium Wars Kane Edition S/N: QX5MR6-V1B1J2M/ IXFX-NRN7-U3RA ———————— sdfvsdfg maya 8,5 Name: fdgdgdf S/N: MUKO dfgdfgdf ———————— Inicom FlashFXP Name: ACME LABS S/N: FLASHFXPQUNNRTA3ugAAAACtW5MNJwXnsl 73nZlbtQgcBJraVoQtY4MYuOMWERAAQQZY+ cXSZ0EAkQyj6kkLIEHDaQIKhs3ZfP9Ls54XBA7/ gQCF7el7iEIINMXzIwtZTMZSLutP74WwrlLm+9 PnW3njFlGLox2gyWBAWuCpFg/p6do9Eaj20M 7yF3JhxhI40h7sv4bU+iBeXrZLZA7ebz9xXjwPIt qVocdTztbzaNfHOWd+Kb84wcptCs————— ——— FileMaker, Inc. FileMaker Pro Advanced S/N: 36FCEBFA54A8FDD3-A6C11 ———————— EA-Games Command and Conquer 3 – Tiberium Wars Cnc3 v1 S/N: NTEW-DXD7T3S5-B1A5-M3Q0 / AB3K-T7EY-K0H6-B4M7O6O7 / S34U-S6GN-O3EA4-P2G8 / ADUCRTBJ-S6K8-S6U8-Y1A5 / QYH9-FUQX-Y0C2M3F5-V0B0 / KAFF-EDVY-H2R8-A5E6-F4B8 / GRL4-PUVZ-K8KV7-T8J8 / NTWNTLG6I1-D3Q5-H0B7 / IG4C-E8AS-M6V1-B8S2B5Y3 / ———————— Xilisoft Xilisoft 3gp video converter S/N: S/N: Name: Free Verion S/N: F-7B4DC02 ———————— slysoft AnyDVD & AnyDVD HD S/N: REGEDIT4 [HKEY_LOCAL_MACHINE\Software\SlySoft\An yDVD\Key] “Key”=”0fw97UItd8HRWy9tYnIwJQiaPoCmhpI Z4f5fUEfYBZU7mZbNkQTIjPx5+WtSpRbnZTOH 2AzcIlSo5TgvyPsrbBEmQs8vOLL3loL6QZ4M0L yjHU1plK0mHFJC82zdM8OwEfep4w8i5tkgyfi1 v7igWSiaaNWWTnt20K/dtWdIgbE7yW0ZsaQ OsPrkG4 manicapital.com EA Games The sims 2 seasons Name: EA Games S/N: A-PPWQ-X2AZ-ZXRLD ———————— new working serial for the sims 2 seasons Smart Projects ISOBuster S/N: Email address : [email&#;protected] Registration ID: CH5TWFYPGZZ5HBXP Serial: 0FEEEEEC5F9ADF7FCFBA7A Email address : [email&#;protected] Registration ID: CH5TWFYPGZZ5HBXP Serial: 0FEEEEEC5F9ADF7FCFBA7A ———————— ELBY Clone CD Version 4 S/N: ———————— Authorization number: ———————— Google sketchup Pro Name: Animalfriend S/N: S/N: KAAAC ———————— xltek AVG Antivirus S/N: THXMV1-PLCSM2ZG-SKU-N3KV THXMV1-P4-CSC-2AM-KWQF THXMV1-PL-CSM2ZG-SKU-N3KV THXMV1-P7-CSDP5S-ZUNNI THXMV1-PP-CSMXQE-YKA-7CI6 THXMV1-PJ-CSIJUK-NQR-H2G TTSOA-P4-CXSK1 TH ———————— THQ Titan Quest Immortal Throne release S/N: ACD0F-FAFEED6 ———————— ? Supreme Commander ? S/N: SR9H-WXZbDR6X-C4GS-X1VB ———————— Microsoft Windows XP Professional Volume License S/N: V2CMK7JD-3R89F-D2KXWVPK3J ———————— none Amust Registry Cleaner Name: REVENGE CREW S/N: fe38ab6fffa6dba64ebe2a62bdccc3e ———————— n/a FlashFXP S/N: FLASHFXPvwCYA16pvwAAAAC2WpcNKwTnwb veeFsasruCD7nT NEqX/qsvG2UWCyyzmq8cYPqQAZVLclU3WLP 9a1BBDpcCDPBi37 v4mlDCL0hAL/D+yVcY1xiEyEGWJ9msVQ9BN1 PN8n9o5TtxNH6G PsKdTdjSJqvksIQfOMeVcG/KngWSJxpEAq PTLU0kJSkjKktd vdUni+hN2AQVCxco22m/XzEyVMSz34G2eBU 55WXffnsVnfO ———————— qewqe windows 10 S/N: qqq ———————— shole ENT modisa workout plan Name: skueeza S/N: ———————— TRABAJO POWER TRANSLATOR 9 S/N: manicapital.com ———————— SEGA Medieval 2 – Total War II S/N: S3U6W8R9-D26N-X34L ———————— Microsoft Expression Web S/N: DDWJCVFGHJ-7GFKQK3D-PFTHW ———————— Genie-Soft Genie Backup Manager Pro S/N: GMP7-EBD9BC-0EDE46E58CF0 ———————— Microsoft Microsoft Office Visio Professional S/N: H9WFF-BVJ3T-2KHD9-HBPP46B ———————— Lexware Lexware Buchhalter V. 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PowerISO Name: Tyrox S/N: 8KBUC-L1JT2-NUYA5-JPD5M-E3RY6 PhotoShop PWWR ClickTeam Multimedia Fusion 2 S/N: NVBAJ24RNUJ84K-T6V3CZ Adobe Photoshop hajro DFX Audio Enhancer 8 S/N: OK08OB05 Gamehouse Scrabble Name: Danmaninsane S/N: VSRTNAFBETFAADA enter serial only when offline Macromedia Director SN:WDW Macromedia Flash v ACDSee v (C) ACD System FLW Macromedia Freehand 10 Page Maker 5 for Win Power Quest Partition Magic FHW begin_of_the_skype_highlighting end_of_the_skype_highlighting pmeneu Delphi WinBoost GOLD Edition Corel Draw 9 WB Registration Code Serial#: [Key #:fexx0] User Name: TheBrabo DR 5T5K4-DVZ7A-AC4T krack rekso translator WindowBlinds Enhanced Serial Number : RT Name : Team BROKEN Password : ar Serial : WB-cagd System Mechanic Pro vf Delphi Unser ID : ^XmoenseN^ Serial Number : P DivX Pack manicapital.com: 03ACABAA0 Active ScreenSaver Builder Name : ^XmoenseN^ SN : 03EDBCA92 AquaReal AREFS3D0AJ-CE1J Need for Speed – Most Wanted SN :P LNKML-S49X-DR7W-6MY9 Condition Zero SN : 58V2E-CCKCJ-B8VSE-MEW9Y-ACB2K WarCraft III SN : MNZBKJ-HIP9-R26ODP-GL3KCR8W Partition Magic SN: PMFR Visual Basic 6 CD key : ACDsee 6 PowerPack SN : ACD Pro Abby Fine Reader 9 FPXF FPXF —————ACDSee Pro D2BDVHTMKHY-3JQ5RV8 D5WDVHT-3YBSYQ-K7WK —————Ace Utilities Pauline Clark 0QGDC11BMJ9W1-AT1V4N-8RHMB3 —————Adobe Photoshop CS3 Mac OSX —————Advanced System Optimizer David Louis 0Z15YH-C41M2U-T7GVVG-YCA0PB-6NP01H —————Agnitum Outpost Security Suite PRO ——— OUTPOST KEY BEGIN ———0xmXh3psv8QjXan2kClW5OjUH9M3cPs /rIzHY /bmjt/A/0r4OjrGvE1ljeTitsvGoUrQYP3b4SyfJ2 V kBxMA6bOnxWpLKOhV4ZQRlUI6dZ0uldH3KO 7yEDPcL qhG0KFDmTUOqZ+3wV0OacgAaDeQSrEewAIc KMi3uhT 32UeFQqV4q/fjSLu86r3yX/gXdWdGRayYXw== ———- OUTPOST KEY END ———– —————AMS Photo Effects AT4RE HK4SAUTRE34R5T5REGHTFIRGTFR8ET4 TEAM DVT FFDFDJFFJDFJD35DHR3FJ39FDFD8FH38F —————Apex Video Converter Super any name FBA19A51EC11AF99BFA 4AEC6 9A50DB6B3D98BD48C86FFE35AB 0BAFF00D 3CA5BCD3E1FEC23DCD3 27E46CA6D CBBB0BADBA6AFF2FFCDA CAF54B7F1 A27CBBEFAAEA8DEFF0EE1 A1A1CAB2B CCEF4FA31C6DD08FA6 8C5EAE90B6E2 C35BBD9CE5CB 8AB89B ADBEEEDA09F0E46DCAF6F5A89 6C8F1FDF19C0 60BFAABF0E4E1BDBBA43 0EA C6ABDAA15EAB5DDBCB0C3 2D4C7DACC65CA —————ASHAMPOO AUDIO STUDIO AMS3AAY4D6-IYKD5P —————Ashampoo music Studio Sam Lenard AMS3AUESFQM Foad Filt AMS3AKTFC99G —————Auto FX Software Mystical Lighting M —————Babylon v6 Che Guevara QZDSEBF94P3K5AUVS8NC6T Rochelle Kessler X9TKT2UWV66SZ4W2R8WKMPT —————Babylon Pro v 1. uninstall previous version then delete babylon folder. 2. reboot manicapital.com installation use this User Name: ieccorp License Code: 9MGGMD72WVV3RFJER94XWVHYT manicapital.com the patch supplied et voila!!! Heiko Koese 4EB76A-8FEB2C-D41FB9-CC4FCE —————Disk Washer ABBCDDISKW —————manicapital.comv [email&#;protected]

Источник: [manicapital.com]
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  • TSID#

  • ADOBE ACROBAT

  • KWWR

  • ADOBE AFTER EFFECTS V

  • EWWR

  • ADOBE DIMENSIONS V

  • DAWR or DJWR

  • ADOBE FRAMEMAKER V

  • EB98

  • ADOBE FRAMEMAKER + SGML

  • F8EC6A

  • ADOBE GOLIVE V

  • GJWR

  • ADOBE GOLIVE V

  • GJWR

  • ADOBE ILLUSTRATOR V

  • ABWR

  • ADOBE ILLUSTRATOR PL

  • ABOR

  • ADOBE ILLUSTRATOR V

  • When it asks for a verification of

  • previous versions choose "Adobe Illustrator " and then

  • Search a specific folder for prior installation.

  • Ulead MediaStudio Pro v Serial :

  • Ulead MediaStudio Pro v Multilanguage Serial :

  • Ulead DVD MovieFactory v Serial :

  • Ulead DVD Picture Show v Serial :

  • Ulead DVD WorkShop v Serial :

  • Ulead GIF Animator V Multilingual Serial :

  • Ulead Photo Express v Serial :

  • Ulead PhotoImpact v Serial :

  • Ulead Video Studio v Serial :

  • Ulead Video Studio v Serial :

  • Unis?ntese Curso de Direito BR Serial : US

  • Universal Translator Serial : KRAY3QT

  • Vecta 3D Serial : IWKS/V3DS-7F9SL1-XAWLEEL0D6N

  • Veritas RecordNow MAX Platinum v Serial : SGW0C-DW0BZ-EEJWR-PH-ETPZM

  • Serial : SGWOC-DW0BZ-EEJWR-PH-ETPZM

  • Visio Professional Edition Serial :

  • Microsoft Visio BR Serial : KW77G-TBTQV7VDWPVDJ

  • Microsoft Visio Serial : dy6wq d3fyg v89by 8kpg9 8yw9m

  • Veritas Volume Manager For Windows v Serial : IJPZ-PPRK-NIB3-J6PC-IWGP

  • Veritas Cluster Server v For Windows Serial : NJZZ-XCL3-VPZX-EFX6P-PNPVisual Cobol Serial : nqnpg-3rj

  • Vue D?esprit v Serial : VUER / VUEF

  • Vue D?esprit v Serial : VUER

  • WinDVD v BR Serial : NJZB3VEIWTK6RGT

  • WinDVD v Serial : YBHPK6AXK7A8R5D

  • Xara3D v Serial : X3D

  • Xara Webstyle v Serial :

  • Xara WebStyle v Serial :

  • Webtrends Analysis Suite vB Advanced Edition Serial : HC-EGE-4HKB-2bME

  • WinTranslator v Licensee: PARADOX

  • English <> German RegCode: xx

  • French <> German RegCode: xx

  • Spanish <> German RegCode: xx

  • Italian <> German RegCode: xx

  • The program will start and will ask for license codes for dictionaries used: License Codes For Dictionaries:

  • Deutengl/Engldeut: xx

  • Deutspan/Spandeut: xx

  • Deutital/Italdeut: xx

  • Deufranz/Franzdeu: xx

  • ACDSee : SpiritMaster,

  • Adaptec Easy CD Creator :

  • Adobe Acrobat : AOWR

  • Adobe Dimensions : DJWR

  • Adobe Golive : GJWR

  • Adobe Illustrator / (Upgrade) : ABWR

  • Adobe Illustrator / (Upgrade) : ABWR

  • Adobe Illustrator / (Upgrade) : ABWX

  • Adobe Illustrator / (Upgrade) : ABWX

  • Adobe InDesign 1 : IPER

  • Adobe Photoshop : PWWR

  • Adobe Photoshop : PWWR

  • Adobe Photoshop : PWWR

  • Adobe Photoshop : PWWR

  • Adobe Photoshop : PWWR

  • Adobe Photoshop : PWXR

  • Adobe Photoshop : PWXR

  • Adobe Photoshop : PWXR

  • Adobe Photoshop : PWXR

  • Adobe Photoshop : PWXR

  • Adobe Photoshop : PWWR

  • Adobe Photoshop : PWWR

  • Adobe Photoshop : PWWR

  • Adobe Photoshop : PWWR

  • Adobe Photoshop : PWWR

  • Adobe Photoshop : PWWR

  • Adobe Photoshop : PWWR

  • Adobe Photoshop : PWWR

  • Adobe Photoshop : PWWR

  • Adobe Photoshop : PWWR

  • Adobe Premiere : MXXR

  • Adobe Streamline : SBWU

  • Andromeda Shadow : 8P

  • AstroWorld Suite c : EAP

  • Asynchrophobia :

  • Banner Maker Professional :

  • Bio97 : ASTAGA (D4C),

  • Blade Pro :

  • Blade Pro :

  • Blade Pro :

  • BootMagic : PMENWDL

  • CacheX For IE : SpiritMaster, CHFJAJCD

  • CD Box Labeler Pro : N?v: Keldo K?d: GPS

  • Chesspartner :

  • ChromaPix 15b : TRPS ,

  • ClipCache a : E65FEDB7D

  • CompuPic , All Latest Builds : SpiritMaster, (phone) , 3CSG02VWS5

  • Crusher : SpiritMaster,

  • Crusher : CZY,

  • Terminator : SpiritMaster, akX16

  • Pal c : SpiritMaster,

  • Copernic Plus99, :

  • Copernic Plus99, :

  • CPU Cool :

  • Customizer for Win98 :

  • Disk Clean Up :

  • DNS Expert : DXPWjrhzwiy

  • Draw4U : SpiritMaster,

  • Dreamveawer : DWW

  • Drive Copy Pro : DCENCD

  • Drive Image Pro : DPENWSCD

  • Easy CD Extractor 3 : SpiritMaster,

  • Easy CD-DA Extractor : N?v: Dexter K?d: EZCDDAXEB2B

  • Easy CD-DA Extractor Build : CZY, EZCDDAX3-E0F35BC1-C9EA2E

  • Easy Tarot : SpiritMaster,

  • Easy : ET*

  • File Chopper : Tex,

  • File Protector : Any Name/manicapital.comDA

  • Fractal Design Poser : FARAUYDAQ

  • Genesis VFX : Serial number = , Authorisation Code =

  • GetRight :

  • GetRight :

  • GetRight :

  • Hardrive Mechanic : 65B

  • Headline Studio : HF01CNCEYK

  • Image Converter :

  • Image Optimizer : ciqui

  • Install Console Developer : N?v: L!M!T [TEX] Jelsz?: MOGYXGQRKHJ

  • Install Shield Pro :

  • Installer Vise : N?v: Kooky [HERiTAGE] K?d: IVRW

  • InstallShield : ISCSLTEMP

  • Intellihance : IWE

  • Intellihance : IDE

  • InternetPhone : V1Q7-SCNNNN-LXBFF/A2BE

  • Invisible Pro : FFFAAA-BFBF-FFAA-FBFB

  • Jammer , Build : Shaligar^Lash, DC

  • c44js

  • c44js

  • JPG Optimizer : AY

  • Kais Power Tools / Retail : KF50CCDJEQ

  • Kais Power Tools / Retail : KPT-WDE-R

  • KPT Gel : KG50WCDNAY

  • Kremlin :

  • Lost & Found :

  • Lost & Found :

  • Microsoft Office Premium :

  • Microsoft Office : MP4F9-W6C8V-HTCCT-T7M7R-Y7K3Y

  • MIRC : SpiritMaster,

  • NetBus Pro : SpiritMaster, 1AAC6DD8

  • NeuroTran ( j?lius 22) : E6L-7LF8N-KCZHP

  • NewsGrabber : Walhalla/oDT,

  • Num. Star Reader : LMs43Fu8/ (upgrade to v )

  • Offline Explorer : CHENZY, XP

  • Painter 3D : FWNAZFPPJ

  • Painter 3D : VARAZCELW

  • PanOpticum Fire : Siege vagy ,

  • Partitition Magic : PMENWDL

  • Partitition Magic : PMDECD

  • Password : Regisztr?ci?s k?d: magicpassword

  • Password : N?v: Sempai^LasH K?d:

  • Password Administrator : N?v: Cissie Goldstein K?d: CBBDA

  • Password Administrator : N?v: DzA kRAker K?d:

  • Password Agent : N?v: i2k = IMYke - @ - K?d:

  • Password Assistant : N?v: !User K?d:

  • Password Bank Pro : N?v: Azrael C?g: Phrozen Crew K?d:

  • Password Keeper : N?v: n03l C?g: Faith98 K?d:

  • Password Manager : N?v: MANiFEST K?d:

  • Password Power : psk1

  • Password Protected LockUp : TNT!PPLF

  • Password Tracker Deluxe : N?v: frenzy K?d: PT

  • Password Tracker Deluxe : N?v: i2k = IMYke - @ - K?d: PT

  • Photoanimator : SpiritMaster, KundaliniRiders Inc., HCI

  • PhotoFrame e : The Brabo, Brabo (comp.), BCE

  • Photoframe Final : BDE

  • Photoframe Final : BDI

  • PhotoJazz : SpiritMaster, Private, MDW2-A5RR-AF3ZJ

  • PhotoSee Pro : SpiritMaster,

  • PhotoTools e : HCI

  • Plugin Manager : LOMAX, KGSIICNET

  • Poser 4 : XF83WBDUSE

  • QuadSucker Web : SpiritMaster, wfqbpqbu

  • Quick Time 4.x : N?v: i2k = IMYke - @ - K?d: 0BE2FF5A

  • Quick Time Pro : N?v ?s C?g: i2k = IMYke - @ - K?d: C-D4EEF-8BEA

  • Raydream : SW

  • RAYflect Phototracer x : PTP-Wabt07wk-pg0qh3a0ajw2

  • manicapital.com build : Alfred-EXE, RS

  • Saved Game Manager b : N?v: i2k = IMYke - @ - K?d: OXvL1r-ueZteR6K

  • ScreenSaver Builder : N?v: i2k = IMYke - @ - K?d: FF65ETNO90FCDF

  • Serials : hackthis

  • Software Organizer Deluxe : N?v: The Exterminators K?d:

  • StealthApp :

  • Teleport Pro : SpiritMaster, Private,

  • The Cleaner x : What you want,

  • The Matrix ScreenSaver :

  • Transparency : WTLE

  • TrayIcon Menu : Site Licence N?v: Crudd [TeX] K?d: CN

  • TrayIcon Menu : Single User License N?v: Crudd [TeX] K?d: BG

  • Tweaki for Power Users : N?v: karpoff K?d: PES

  • Tweaki for Power Users : N?v: i2k = IMYke - @ - K?d: CED

  • Ulead Audio Editor VE :

  • Ulead Video Capture VE :

  • Ulead Video Editor VE :

  • Ulead Video Studio SE :

  • Ultimate Game Cheating System : CS

  • Ultra Edit c : N?v: i2k = IMYke - @ - K?d: ZJTBBXUD

  • Ultra Edit b : N?v: i2k = IMYke - @ - K?d: ZJTBBXUD

  • Universe : draXXter,

  • ViaVoice Millenium Edition : RPA

  • VirtuaGirl : N?v: K?d:

  • WebPhone : Key: HTAJ12D

  • WinAce Final : N?v: i2k = IMYke - @ - K?d: ACEFYWE6EEQ9K9AR

  • WinAmp < : N?v: i2k = IMYke - @ - K?d:

  • WinAmp Skin Maker : N?v: i2k = IMYke - @ - K?d:

  • WinAmp Skin Maker : N?v: i2k = IMYke - @ - K?d:

  • WinAmp Skin Maker : N?v: Szabby [SCT] K?d:

  • WinBoost Gold Ed. : SpiritMaster, 7W5I4-RNVR7Y-A41F

  • WinBoost Standard Ed. : SpiritMaster,

  • WinBoost98 / : CoKeBoTtLe99,

  • Window Blinds : N?v: i2k = IMYke - @ - K?d: WB-1vtfj1

  • Window Blinds Final : N?v: +DzA kRAker SPECIAL LICENCE FOR THE FANS OF WINDOW BLINDS K?d: WB-dfj

  • Window Blinds Final : N?v: TOM&JERRY K?d: WB-bc1ecc90

  • Window Blinds Final : N?v: THE TNT CRACK TEAM K?d: WB-3mc4a8n

  • Window Blinds Final : N?v: +DzA kRAker K?d: WBbd

  • Window Blinds Final : N?v: I HATE WINDOW BLINDS K?d: WB-3mbh

  • Window Blinds Final : N?v: FREE STARDOCK LICENCE K?d: WB-4encpd6

  • Window Blinds Final : N?v: GIGEL K?d: WBd

  • Window Blinds : N?v: Reanimator^LasH K?d: WB-j2e89f9

  • Window Blinds : N?v: i2k = IMYke - @ - K?d: WB-1vtfj1

  • Window Blinds : WBef13

  • Window Blinds : N?v: ChoRdLesS K?d: WBe22c

  • Window Blinds : WBef13

  • Window Blinds a : WBf

  • Window Blinds : WB-dca3

  • Window Blinds Build : N?v: SPeY K?d: WBd61a68d

  • Window Blinds manicapital.com : N?v: Stony Crackers Team K?d: WBmiah

  • WinRar manicapital.com : N?v: sNeaKeR FiVe [] K?d: 5B68D2BEA44C92

  • WinHacker : N?v: Predator C?g: Phrozen Crew K?d: c8d75

  • Windows Logo Changer : N?v/N?v: L!M!T C?g: The Exterminators E-mail:

  • K?d: D6NRVZYRLA0M

  • WinRescue95 , : msR3I8aUi9y2E84L

  • WinRescue98 ,,, : SvetCHRISTA

  • WOW AstrologyForLovers : Mr_GReeN [WkT!],

  • Xenofex : JKGDDBEEKLCH

  • Nero

  • Nero Normal:

  • Nero OEM:

  • Nero MP3:

  • Nero Normal:

  • Nero OEM:

  • Nero MP3:

  • Nero

  • Nero

  • Nero

  • Nero

  • Nero

  • Nero

  • Nero

  • Nero

  • Nero

  • Nero

  • Nero

  • Nero

  • Ulead VideoStudio v serial

  • Adobe PhotoShop - serial:

  • Adobe PhotoShop - serial:PWWR

  • Adobe illustrator 10 Beta Build 76 - serial

  • Adobe Illustrator 10 SN or [/font]

  • HK-F68GKG97XDNC6T

  • Sonic Foundry ACID Style DJ a 7H-ZPLB1HB9H9-BV4X6S

  • Sonic Foundry SoundForge 6 HKYBN9-BMC-SQYMP9

  • Computer ID HLBW

  • Sothink SWF Decompiler 8SJSDK

  • SpamEater Pro Build 91 Name:[ندعوك للتسجيل في المنتدى أو التعريف بنفسك لمعاينة هذا الرابط]

  • Code:0D0D-2FBB-0EFEE7

  • SPAM INSPECTOR for Outlook / () s/n: YT6RJ-KHGMT3L-DC3NX-7Y7UU

  • SPSS 8 ID -

  • Serial -

  • SPSS s/n:

  • SPSS Serial: Skip the code entry

  • Serial: Skip the code entry

  • SPSS Sigmaplot s/n:

  • License Code:

  • S-Spline 2 Z1-G7BGQ-NC2J9-J5FA9-J2JJ7-EFAAC

  • Statistica '97 s/n: SPG51

  • key:

  • Statistica PL key: RVYW4DD5XC5X6X

  • serial: AXAAL

  • Statistica PL Cd Key (standard): NGN51Q55NPN

  • Statistica PL Cd Key (network) : 7G8N5QKHH89

  • NIN:

  • StatSoft - Statistica AX v Key: RVYW4DD5XC5X6X

  • Serial: AXAAL

  • Steganos Security Suite 4 key:

  • Steinberg Clean DLCY9-MS5

  • Steinberg Cubase VST32 Multilanguage

  • Steinberg Mastering Edition v

  • Steinberg WaveLab For Win9X: ycy8hy6uy-eppnby8-yku9

  • For WinNT: ycy8ck8lv-eppnby8-yvmx

  • Steinberg: Get it on CD STTHAN9HY-EPPNBY8-TJC

  • STTHACSX8-EPPNBY8-YDL

  • Studio Biblijne

  • Style XP M MHSW7G-VGNATAAN-KVQATRC5-BCJCDGDJ-4FEFKS

  • Subiekt for Windows PL SERIAL:

  • SubmitWolf Pro Name:Free User Code:E

  • SuperBike

  • Adobe PhotoShop - serial:

  • Adobe PhotoShop - serial:PWWR

  • Adobe illustrator 10 Beta Build 76 - serial

  • Adobe Illustrator 10 SN or

  • HK-F68GKG97XDNC6T

  • Sonic Foundry ACID Style DJ a 7H-ZPLB1HB9H9-BV4X6S

  • Sonic Foundry SoundForge 6 HKYBN9-BMC-SQYMP9

  • Computer ID HLBW

  • Sothink SWF Decompiler 8SJSDK

  • Code:0D0D-2FBB-0EFEE7

  • SPAM INSPECTOR for Outlook / () s/n: YT6RJ-KHGMT3L-DC3NX-7Y7UU

  • SPSS 8 ID -

  • Serial -

  • SPSS s/n:

  • SPSS Serial: Skip the code entry

  • Serial: Skip the code entry

  • SPSS Sigmaplot s/n:

  • License Code:

  • S-Spline 2 Z1-G7BGQ-NC2J9-J5FA9-J2JJ7-EFAAC

  • Statistica '97 s/n: SPG51

  • key:

  • Statistica PL key: RVYW4DD5XC5X6X

  • serial: AXAAL

  • Statistica PL Cd Key (standard): NGN51Q55NPN

  • Statistica PL Cd Key (network) : 7G8N5QKHH89

  • NIN:

  • StatSoft - Statistica AX v Key: RVYW4DD5XC5X6X

  • Serial: AXAAL

  • Steganos Security Suite 4 key:

  • Steinberg Clean DLCY9-MS5

  • Steinberg Cubase VST32 Multilanguage

  • Steinberg Mastering Edition v

  • Steinberg WaveLab For Win9X: ycy8hy6uy-eppnby8-yku9

  • For WinNT: ycy8ck8lv-eppnby8-yvmx

  • Steinberg: Get it on CD STTHAN9HY-EPPNBY8-TJC

  • STTHACSX8-EPPNBY8-YDL

  • Studio Biblijne

  • Style XP M MHSW7G-VGNATAAN-KVQATRC5-BCJCDGDJ-4FEFKS

  • Subiekt for Windows PL SERIAL:

  • SubmitWolf Pro Name:Free User Code:E

  • SuperBike

  • Super Copy manicapital.com

  • Code

  • SuperCleaner

  • SuperHTTP Name: laxity s/n:

  • Name: PNT s/n:

  • SurfOnGuard manicapital.com

  • Code

  • Sygate Personal Firewall Pro bS Sn:GWU

  • Cd:QLW4GXLV

  • SySpeed Name: CD

  • s/n:

  • System Cleaner Name: FCN98 [NighTHanG]

  • s/n: 5A38AC9B4BG52CE

  • System Kodowy Nazwa użytkownika : MAXIBOGAS

  • Kod systemowy : 7TEAM

  • Kod aktywacyjny : BESNEELMUNMCTEID

  • System Mechanic h Name: Crack Demon

    1. Industrial code: ND

  • T-
  • Talisman Name: FiGHTiNG FOR FuN
  • s/n:
  • Teleport Pro manicapital.com
  • Code
  • Terminarz Nazwa użytkownika : MAXIBOGAS
  • Kod systemowy : 7TEAM
  • Kod aktywacyjny : BQGPWGXYPGPQUNYH
  • The Bryce 3D 2
  • The Cleaner build name: 9down
  • kod: B3-BCD
  • The Cleaner Build manicapital.com
  • Code:3A4C-A9FE-A9BFA
  • ThumbsPlus Name:king_KINK@manicapital.com
  • Code:dc6r58p9ymrzy
  • Tiger 98 n:Zomo
  • s:CP manicapital.com
  • ser:
  • pass: 12OcO45MM9Q8OO
  • TMPGEnc Plus CODE: TEV3TPRRBV-DCAT
  • CODE: TEPJ4HH-2JEHZV
  • TMPGEnc TEV3TPRRBV-DCAT
  • TMPGEnc s/n: TEV3TPRRBV-DCAT
  • TMPGEnc Plus s/n: TENC9E-RKLNB7-DWIV
  • TMPGEnc Plus s/n: TEPKNB-NPPC-5CK8-GJXD
  • Topee CD Ripper manicapital.com
  • FF63BCEC49AFCC0
  • Traktor Dj Studio
  • Tray Helper manicapital.com
  • Code
  • Truespace SSE
  • TuffTest Pro
  • TuneUp Utilities Name: Black Killer
  • Company: manicapital.com
  • s/n: GPDTJ-PTWEPDXCQ-UC-FDUU3
  • TweakMASTER Pro Build rEGNAME: VDOWN@NET
  • rEGCODE: N1E31DCC5BD
  • codeE88EFBD-DB7C4
  • Tweak-XP Pro ID: E
  • Tweak-XP Pro ID: E
  • -U-
  • Uczę się muzyki PL SPMUZ
  • Układ Okresowy Pierwiastków E2
  • Ulead Cool 3D v
  • Ulead DVD Movie Factory v
  • Ulead DVD Movie Factory 2 s/n:
  • Ulead DVD PictureShow 2 s/n: A
  • Ulead Face Factory s/n: RAE
  • p/n: A
  • Ulead Gif Animator 5 s/n: GJWR
  • Ulead MediaStudio Pro
  • Ulead MediaStudio
  • Ulead MediaStudio Directors Cut
  • Ulead MediaStudio Pro s/n: ADCN4
  • Ulead PhotoExpress Platinum
  • Ulead PhotoImpact
  • Ulead VideoStudio
  • Ulead VideoStudio Serial:
  • UltraEdit b THG
  • U0Z7C-T5ZF3K-C9F7E
  • UltraISO manicapital.com
  • Code:4AAEF-5FBB7
  • Universal Translator KRAY3QT
  • -V-
  • VentaFax & Voice Name: RZ
  • s/n: E
  • VentaFax & Voice build 14 ID:
  • SN:
  • Virtual CD v HD4A-B8ACF9
  • HFB
  • HFAAFF
  • s/n: NNAAB3
  • VirtualDrive VDP
  • Visio Enterprise
  • Visio Professional
  • Visio Standard
  • Visio Pro
  • Visio Pro dy6wq d3fyg v89by 8kpg9 8yw9m
  • Visual MP3 Name: TEAM VIRILITY
  • Code: 4F5A
  • Visual Studio .NET s/n: T7KXGHXC-JYRFVHDM7M
  • VMware Workstation v for Linux s/n: 68N2DXFHEKPUT
  • Voice Changer Software Email:cdrem@manicapital.com
  • Name:cdrem
  • Serial Number
  • Vue d'Esprit 4 VUEO/
  • VUEE
  • Vue d’Esprit Install: VUER
  • s/n: VUEF
  • -W-
  • Wave Corrector name: Reanimator^LasH
  • code: TMRKEYQM
  • Wave Corrector manicapital.com
  • Code:RESBALTS
  • WebMenu Studio manicapital.com
  • Company Name:RussianWarezTeam
  • Code
  • WinAce Name:best
  • Ser:ACEBPAR8JZ5YZWIQHTAUCZA2
  • WinBoost s/n: bh3y6q-6k7gb4rr5g36
  • WinBoost s/n: hy7k8r-4a7jp4my4s46
  • s/n: cu3i4m-3y8vg6dq3x76
  • WindowBlinds v Enhanced WB-I2RIR-B0JDL-N3EKQFML-OGD9G
  • Windows Accelerator s/n:
  • Windows Server QW32KT2T-3D2PJ-DXBWY-C6WRJ
  • Windows System Optimizer Name: use any name
  • Code: 4EJ-FpQEFE-JIU
  • Windows XP Plus Digital Media Edition K2QX9-FPG6H-FY2K3-MF4PC-7QT8M
  • Win DVD 0KTAX20ZTOS2IGS
  • WinDVD GHT7C7R84E0
  • WinDVD RVEE5DD-NIIMF1UC90SL-Q0TQBVVA
  • WinGate Name: Silentio
  • s/n: 40VMFSC6IIIV0HKR0
  • WinGate Name: Free User
  • s/n: JQBLELWNC2L45MV21
  • WinGuard Pro License Code: dsgl
  • WinGuard Pro - Premium edition Name:Vepergen // TSRh
  • S/n:8fhr0gf4-gmvnm
  • WinGuardian Pro Name:fxyang
  • s/n: dsgl
  • WinOnCD NAME: GLoW Company: GLoW
  • s/n: m
  • WinOnCD Power Edition K
  • WinOnCD Power Edition e
  • b
  • WinOnCD Power Edition OB-PROBS-QV7NK-SNGWH
  • WinOnCD Plextor L
  • WinRAR iwan / CrackPL
  • 8DB88AABF9A5A3F0
  • Code: C8ABB64E
  • WinWap Pro s/n: WWAA6
  • WinXP Manager Code::R9ST9-ELIM6-ETUU2-RDGFX
  • Code:B36UO-ELIMSBTS-9F0BI
  • WinZip Name: x Code:
  • WinZip beta build manicapital.com
  • Code:A6EB1A42
  • Word Translator EP2 N3 K6WRM
  • Wszystko o seksie
  • X-
  • Xara 3D
  • Xara 3D v s/n: X3D
  • Xara Webstyle s/n:
  • Xara Webstyle s/n:
  • X****l s/n: SQXM2 00F24 9D
  • Code:
  • ABC Toolkit v for Windows :

  • ABC Toolkit v for Windows :
  • Источник: [manicapital.com]
    ACID Style DJ 3.0a serial key or number

    Essential metabolism for a minimal cell

    Abstract

    JCVI-syn3A, a robust minimal cell with a kbp genome and genes, provides a versatile platform to study the basics of life. Using the vast amount of experimental information available on its precursor, Mycoplasma mycoides capri, we assembled a near-complete metabolic network with 98% of enzymatic reactions supported by annotation or experiment. The model agrees well with genome-scale in vivo transposon mutagenesis experiments, showing a Matthews correlation coefficient of The genes in the reconstruction have a high in vivo essentiality or quasi-essentiality of 92% (68% essential), compared to 79% in silico essentiality. This coherent model of the minimal metabolism in JCVI-syn3A at the same time also points toward specific open questions regarding the minimal genome of JCVI-syn3A, whicSynthetic Mycoplasma mycoides JCVI-syn clone sMmYCp, complete sequenceh still contains many genes of generic or completely unclear function. In particular, the model, its comparison to in vivo essentiality and proteomics data yield specific hypotheses on gene functions and metabolic capabilities; and provide suggestions for several further gene removals. In this way, the model and its accompanying data guide future investigations of the minimal cell. Finally, the identification of 30 essential genes with unclear function will motivate the search for new biological mechanisms beyond metabolism.

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    eLife digest

    One way that researchers can test whether they understand a biological system is to see if they can accurately recreate it as a computer model. The more they learn about living things, the more the researchers can improve their models and the closer the models become to simulating the original. In this approach, it is best to start by trying to model a simple system.

    Biologists have previously succeeded in creating ‘minimal bacterial cells’. These synthetic cells contain fewer genes than almost all other living things and they are believed to be among the simplest possible forms of life that can grow on their own. The minimal cells can produce all the chemicals that they need to survive – in other words, they have a metabolism. Accurately recreating one of these cells in a computer is a key first step towards simulating a complete living system.

    Breuer et al. have developed a computer model to simulate the network of the biochemical reactions going on inside a minimal cell with just genes. By altering the parameters of their model and comparing the results to experimental data, Breuer et al. explored the accuracy of their model. Overall, the model reproduces experimental results, but it is not yet perfect. The differences between the model and the experiments suggest new questions and tests that could advance our understanding of biology. In particular, Breuer et al. identified 30 genes that are essential for life in these cells but that currently have no known purpose.

    Continuing to develop and expand models like these to reproduce more complex living systems provides a tool to test current knowledge of biology. These models may become so advanced that they could predict how living things will respond to changing situations. This would allow scientists to test ideas sooner and make much faster progress in understanding life on Earth. Ultimately, these models could one day help to accelerate medical and industrial processes to save lives and enhance productivity.

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    Introduction

    Establishing the core requirements of cellular life is a fundamental challenge of biology. The question of the minimal set of biochemical functions necessary for a cell to grow and replicate has been studied from a number of angles for more than 20 years. It has long been suggested (Morowitz, ) that a model to study the basics of cellular life would be the mycoplasmas—a group of bacteria with small genomes (– kbp (Herrmann, ; Fraser et al., )) lacking a cell wall, which evolved via extreme genome reduction from low GC content Gram-positive ancestors (Pollack et al., ). Mycoplasmas exist as parasites or saprotrophs and are adapted to scavenging nutrients and cellular building blocks from their niche environments, which enabled them to lose many metabolic capabilities.

    The genome of the human urogenital pathogen Mycoplasma genitalium ( kbp, genes overall, for proteins, 43 for RNAs), sequenced in (Fraser et al., ), is the smallest genome of any autonomously replicating cell found in nature and has thus been deemed a close approximation to a minimal genome (Glass et al., ). In particular, different efforts have been undertaken to establish a minimal set of genes based on the near-minimal M. genitalium genome. A comparison of the first two sequenced bacterial genomes (the Gram-positive M. genitalium (Fraser et al., ) and the Gram-negative Haemophilus influenzae (Fleischmann et al., )) yielded orthologous genes that were suggested to approximate a minimal set of bacterial genes (Mushegian and Koonin, ); a subsequent comparative study, including genomes from several free-living and endosymbiotic bacteria, proposed a minimal set of genes (Gil et al., ). A limitation of this approach lies in the possibility of the same function being fulfilled by non-orthologous proteins in different organisms, in which case it would not be captured by searching for orthologous genes. Transposon mutagenesis studies (Hutchison et al., ) avoid this drawback by directly probing the dispensability of individual genes in a single organism via random gene disruption, and testing for viability. In M. genitalium, such studies have suggested out of the protein-coding genes to be essential (Glass et al., ).

    An important limitation of deriving a minimal gene set from essentiality information on individual genes lies in the fact that more than one gene can fulfill the same function, and while neither gene is essential individually, at least one of them has to be present in a functional minimal genome. Thus, while removal of either gene would be nonlethal, removing both would create a synthetic lethality. This problem can, in principle, be circumvented by sequential gene deletion starting from a given wild-type organism (as partially done for Escherichia coli and Bacillus subtilis (Juhas et al., ; Pósfai et al., )), with testing for viability and growth rate after each deletion. In principle, this would not only yield the information on a minimal genome, but also would produce a living organism controlled by such a genome. However, the time and resource costs of minimizing a genome by serial deletion of dispensable genes are prohibitive.

    In , we developed a successful bottom-up approach to design a minimal genome and create a living cell controlled by it (Hutchison et al., a). Starting with the gene sequence from the kbp genome of the ruminant pathogen Mycoplasma mycoides capri serovar LC GM12, a minimal genome of kbp was designed and constructed containing genes ( protein-coding genes and 35 genes for RNAs) (Hutchison et al., a). The resulting strain, JCVI-syn (NCBI GenBank: manicapital.com (Hutchison et al., b)), has a genome smaller than that of any independently-replicating cell found in nature and is considered to be our ‘working approximation to a minimal cell’. This achievement was the culmination of a series of breakthroughs in synthetic biology. In , the successful transplantation of an M. mycoides capri LC GM12 genome into a Mycoplasma capricolum recipient cell was reported (Lartigue et al., ), transforming the recipient cell to the species of the implanted DNA. In , the complete genome of M. genitalium was synthesized from scratch, starting with chemically synthesized oligonucleotides and stepwise recombination in vitro and subsequently in Saccharomyces cerevisiae (yeast), using the available genetic manipulation tools to assemble the genome as a plasmid inside the yeast cell (Gibson et al., ). These methods enabled the construction of JCVI-syn, the first cell controlled by a synthetic genome (NCBI GenBank: manicapital.com) (Gibson et al., a; Gibson et al., b). This was accomplished by synthesizing of a copy of the M. mycoides capri LC GM12 genome along with vector sequences that allowed cloning in E. coli and yeast, and its subsequent transplantation into M. capricolum recipient cells to yield JCVI-syn These milestones enabled the synthesis of reduced versions of the JCVI-syn genome with subsequent transplantation into M. capricolum to test for viability. The genome reduction process was guided by transposon mutagenesis studies on the original JCVI-syn genome, as well as on intermediate reduced genome versions. Successful genome minimization depended on identifying both essential genes, whose disruption is immediately lethal, and quasi-essential genes, whose disruption causes an observable growth disadvantage. Quasi-essential genes were identified by observing if cells with potentially growth-reducing gene disruptions were outgrown during sufficiently long competition experiments, so that cells sampled from much later generations no longer contained the disrupted gene. Three cycles of genome design, assembly and growth testing yielded JCVI-syn (Hutchison et al., a).

    JCVI-syn contains all the genes of JCVI-syn that are required for growth. This includes both essential and quasi-essential genes. Individually non-essential genes were removed in the design for JCVI-syn to the greatest extent possible without causing synthetic lethality or a major growth disadvantage from simultaneous knockouts. However, in a few cases, genes that appear to be non-essential were retained for ease of genome design and construction. Intriguingly, the role of a considerable fraction of the minimal genome of JCVI-syn could not be elucidated in spite of extensive bioinformatic analyses. At the time of publication of the minimal cell, of the genes (∼31% of the genome) could not be assigned a completely specific biological function. Assignment to a broad functional category could not even be made for a subset of 79 genes. These genes of unknown or poorly defined function potentially point toward required features of cellular life yet to be discovered.

    The original minimal cell JCVI-syn genome was assembled by combining individually minimized 1/8 chromosome segments (Hutchison et al., a). Phenotypic traits of JCVI-syn included extensive filamentation and vesicle formation during growth, and a doubling time of 2–3 hr (compared to the spheroidal morphology and 1 hr doubling time conferred by the JCVI-syn genome). To address these phenotypic disadvantages, an alternative design of segment six was found to restore consistent morphologic features and increase the growth rate, while retaining a near-minimal genome. This new design incorporated 19 additional genes from JCVI-syn segment six that were not present in JCVI-syn, including those encoding the cell partitioning proteins FtsZ and SepF along with others of unknown function; in addition, two other genes retained in JCVI-syn segment six were removed. The complete genome sequence of this strain, termed JCVI-syn3A, is available through NCBI under the accession number manicapital.com (Glass, ) This entry contains genomic features, however three of those are pseudo-genes and two are genes required for cloning in yeast.

    JCVI-syn3A has a doubling time of ∼2 hr and consistently forms spherical cells of approximately nm in diameter. With a kbp genome containing genes of which code for proteins and 38 for RNAs, JCVI-syn3A still has a smaller genome than any natural autonomously replicating cell while providing a robust and versatile platform to study the basics of life. In particular, this minimal cell opens up the possibility to pursue the construction of a complete in silico model including the function of all genes. The map of protein coding genes (Figure 1) clearly shows the fundamental importance of Syn3A as a platform to study the principles of life. Among the model bacteria E. coli and the related and well-studied (Güell et al., ; Kühner et al., ; Yus et al., ; Maier et al., ; Wodke et al., ) Mycoplasma pneumoniae, JCVI-syn3A has the smallest ratio of genes involved in metabolism to those in genetic information processing. With 91 it also has the smallest number of genes that are considered to have no known (unclear) function compared to and for M. pneumoniae and E. coli, respectively (see Table 1 and Supplementary file 1C–1D for an itemized account of the functional categories for the three genomes).

    A model for ribosome biogenesis that includes DNA replication, transcription, translation, and ribosome assembly in slow growing E. coli has already been developed (Earnest et al., ; Earnest et al., ). As its components have on average 50% sequence identity to those genetic information processing genes in JCVI-syn3A, this model is assumed to be applicable to JCVI-syn3A as well. Hence, the next important step in modeling JCVI-syn3A is the reconstruction of its metabolic network.

    The metabolic reconstruction presented here is based on the curated genome annotation, extensive experimental information from the literature on M. mycoides capri and other mycoplasma species, and accompanying transposon insertion and proteomics data. Our model features reactions organized in nine subsystems (see Supplementary file 1B), involving metabolites, catalyzed by gene products of genes, thus covering a third of the genes of JCVI-syn3A. The reconstruction process enabled us to suggest several annotation refinements and updates, and yielded a metabolic network that is fairly complete.

    Together with the reconstructed biomass composition of JCVI-syn3A and estimates of its reaction flux constraints and energy expenses, the reconstructed metabolic network was cast into a flux-balance analysis (FBA) model (Orth et al., ). FBA yields the set of steady-state reaction fluxes through a metabolic network that maximize a pre-defined objective function, for example production of cellular biomass. The solution space of possible fluxes is constrained not only by the steady-state assumption, but also by specific flux limits accounting for maximal uptake/secretion rates or cellular energy expenses. If these flux limits are not known, the network stochiometry predicts the biomass yield achieved by the cell, that is gram biomass produced/gram carbon source taken up (or equivalently biomass production rate/carbon substrate uptake rate). If flux constraints, in particular substrate uptake rates are known or can be assumed, the yield as growth rate per uptake rate can be converted to an absolute growth rate. While measurements to derive such flux constraints are not available yet for JCVI-syn3A, some measurements are available from other mycoplasmas and bacteria that have the same high-affinity glucose transporter (PtsG) found in JCVI-syn3A. Using the glucose uptake rate measured in M. pneumoniae (Wodke et al., ) (which is similar to the one measured in slow-growing E. coli (Fuhrer et al., )) and other constraint estimates allows us to provisionally predict a growth rate for JCVI-syn3A; this model growth rate is however sensitive to the assumed uptake rate (see Section 'Sensitivity analysis' in Appendix 1). In this article, the growth rate predicted by the model is presented with the understanding that for the aforementioned reasons, the prediction is provisional and comes with a degree of uncertainty. This uncertainty has no bearing on the prediction of in silico gene essentialities (see below), which can be obtained by removing certain genes in the model and their associated reactions, and testing whether FBA still predicts a nonzero growth rate for the resulting in silico knockout.

    This FBA model for JCVI-syn3A allows for the analysis of the properties of minimized metabolism in JCVI-syn3A. In particular, gene essentiality can be compared between the model and experimental transposon mutagenesis data. Random gene disruption by bombardment with transposon insertions (Hutchison et al., ; Glass et al., ) was used to identify non-essential genes in JCVI-syn that to the most part were removed during the construction of JCVI-syn (Hutchison et al., a); here, genome-scale transposon mutagenesis studies were carried out on JCVI-syn3A to survey the individual essentiality of all its remaining genes. We find that transposon- and model-derived gene essentiality agree well, with every in silico essential gene being at least quasi-essential in vivo (i.e. removal might not be immediately detrimental, but give a growth disadvantage). The metabolic reconstruction allows us to rationalize the non-essentiality of some genes, and to propose possible further gene removals in JCVI-syn3A. These suggestions from the model are of particular interest as transposon mutagenesis experiments only probe the individual essentiality of genes and do not yield information on which genes could be removed simultaneously. The metabolic construction, on the other hand, allows us to suggest which genes might be simultaneously removed. At the same time, in silico and in vivo essentiality also show some discrepancies, which lead us to postulate new hypotheses about specific gene functions or metabolic capabilities. Protein expression profiles of essential and non-essential genes, classified by either transposon mutagenesis studies or FBA in silico gene knockouts, were not found to differ significantly–possibly indicating by and large the absence of expression regulation that would discriminate gene products based on their essentiality. Finally, the reconstruction process as well as the gene essentiality comparison have yielded a number of informed hypotheses and suggestions for specific experiments that will guide the ongoing experimental investigation of gene functions in the minimal cell.

    Results

    While the minimal cell JCVI-syn3A is a new organism with little experimental data yet available, its natural precursor M. mycoides capri has been studied in depth, which informed all aspects of the metabolic model. To refer to genes in the JCVI-syn3A genome, we use the locus names of the form MMSYN1_xxxx as used in the annotation of JCVI-syn (Hutchison et al., a) to allow us to discuss genes deleted in JCVI-syn3A more clearly. The MMSYN1_ prefix is omitted for brevity. Understanding the in vivo essentiality of genes in JCVI-syn3A is an important first step to the development of the metabolic reconstruction: this is presented first in Section 'Transposon mutagenesis experiments probe in vivo gene essentiality'. Using the protein expression profiles measured for JCVI-syn3A, the biomass composition of JCVI-syn3A is then derived in Section 'Biomass composition and reaction', as well as the biomass reaction used in the model. The construction and justification of the metabolic model is presented in Section 'Metabolic reconstruction'. The steady-state fluxes obtained from the model are then compared in Section 'Steady state fluxes' to experimental fluxes, as well as to protein abundance-based flux limits. The metabolic energy usage of JCVI-syn3A is analyzed in Section 'Energy usage'. Gene essentiality obtained from in silico gene knockouts is presented in Section 'In silico gene knockouts and mapping to in vivo essentiality'. Finally, we compare protein expression profiles between essential and non-essential proteins as identified in the model or in vivo in Section 'Abundances of essential and non-essential proteins'.

    Transposon mutagenesis experiments probe in vivo gene essentiality

    Transposon insertion mutagenesis studies were performed in order to probe the dispensability of individual genes in JCVI-syn3A (see Section 'Materials and methods'). In this experiment, transposons are randomly inserted into the chromosomes of a population of cells that is then plated under selection for a drug resistance gene carried by the transposon (Hutchison et al., ; Glass et al., ). After transferring to a liquid culture (‘passage zero’, ), four serial passages are performed. DNA from the pooled colonies is isolated and sequenced to determine the location of transposon insertions within the genome at the beginning or at the end of the experiment. When determining transposon locations at the beginning of the experiment, is used over to limit any contamination from the DNA of non-viable cells. The number of insertions observed in a coding region can then be used to infer the importance of that gene. We note that not every insertion will necessarily obliterate a gene’s function. A graphical presentation of the essentiality classification along with the distribution of transposon insertions over a portion of the genome is presented in Figure 2. It shows that secA/ is heavily hit with insertions in the 3' 25% of the gene (but practically nowhere else); however, SecA is certainly essential because it is a necessary component of the protein translocase, which inserts proteins such as transporters into the membrane. While the absence of gene products for genes carrying transposon inserts has not been confirmed experimentally, genes with relatively high insertion counts are more likely to be functionally disrupted and thus non-essential. Genes that are not required by the organism to grow in rich media will contain many transposon insertions (‘non-essential’ genes), whereas genes required for cell viability will be sparsely hit by transposon insertions. To identify genes whose disruption might not be immediately detrimental but might cause a growth defect apparent later on, sequencing of the transposon mutagenesis library was performed on cells as well. Cells with a gene disruption that is not immediately lethal but causes a growth disadvantage will be outgrown after , and the number of insertions for that gene will then significantly decrease from to . These genes are denoted ‘quasi-essential’.

    A Poisson mixture model was used to partition the coding regions into two sets of genes based on the transposon insertion density. By comparing the assignment of genes into classes of sparse and dense transposon insertions between and , essentiality can be inferred. This classification method considers a gene to be essential if it has been classified to have sparse transposon insertions in both and , quasi-essential if it was classified to have dense transposon insertions for and sparse insertions for , and non-essential if the gene was classified as densely hit for both and . See Section 'Materials and methods' for a complete description of the classification method. Figure 2—figure supplement 3 shows the fit of the model to the distribution of transposon insertion counts per gene.

    In six out of instances, the mixture model failed to classify the gene, either due to low assignment confidence or due to increased insertions from to . The short ribosomal proteins S9 (rpsl/), L27 (rpmA/), and L31 (rpmE/) were manually assigned as essential since they are necessary to construct a functional ribosome. The gene secA/ could not be automatically classified since the mixture model predicted the gene to be more heavily hit with insertions in than in ; it was assigned as essential as it is a major component of the translocase assembly. The insertions occurred in the C-terminus linker domain considered to be important for binding to phospholipids and preprotein translocation. A gene of unclear function () was predicted by the model to be essential at a slightly higher probability than quasi-essential ( vs. , respectively); however, it was manually assigned to be quasi-essential following its previous assignment in JCVI-syn (Hutchison et al., a). Thioredoxin (trxA/) was assumed to be essential since its associated reductase (trx/) was predicted to be essential by the mixture model. Only one gene was misclassified: the ATP synthase subunit (atpC/), initially classified as non-essential, was manually reassigned to essential since all other ATPase subunits (atpD/ through atpB/) were essential according to the mixture model. The majority of transposon insertions in atpC/ are found in the region, similar to the pattern seen in secA. However, it is possible that the subunit may not actually be essential since in M. pneumoniae, transposon insertions into the atpC (MPN) lead to viable cells with decreased cytadherence activity (Shimizu et al., ).

    The set of genes classified quasi-essential could potentially include essential genes which cannot be discriminated using these transposon insertion data. For these misclassified genes, it is possible that although expression of the gene product essential for cell growth has been halted, previously translated essential proteins remain in the cell in sufficient quantities to maintain cell growth and division up to . A further discussion of this argument is presented in Section 'Completeness of the model' and Appendix 1.

    The genes identified as non-essential by the Poisson mixture model may contain ‘weakly’ quasi-essential genes, that is disrupted genes which confer a minor growth disadvantage. This behavior would manifest as a decrease in transposon insertions between and , but not such a steep decline that the gene is observed with little to no insertions. To identify these ‘weakly’ quasi-essential genes, the genes classified as non-essential were subjected to further classification using -means clustering of the ratio of the number of transposon insertions in to assuming two clusters (see Figure 2—figure supplement 3). Of the genes initially classified non-essential, 42 were reclassified as quasi-essential.

    The assignment of essentiality classes and distribution of transposon insertions over the entire genome are presented in Figure 2—figure supplements 1,2, and Supplementary file 3. Genomic positions of transposon insertions are listed in Supplementary file 2. Figure 3 summarizes the breakdown of the essential, quasi-essential, and non-essential genes according to the functional classes. Of the coding genes in JCVI-syn3A, 60% are essential, 25% are quasi-essential, and 15% are non-essential by this analysis. The detailed breakdown of the JCVI-syn3A genome into these classes (Table 1) shows that of the 91 genes of unclear function, 30 are essential, 32 are quasi-essential, and 29 are non-essential. Those 30 essential genes could represent new biological mechanisms not yet defined and should motivate the search to discover their function (Alberts, ).

    Since on average only one transposon insertion occurs per cell and the identification of insertion locations within the genome is performed over an ensemble of cells, these transposon mutagenesis studies can only reveal individual gene essentialities. To probe the essentiality of groups of genes, one would need to perform targeted multiple knockout studies. However, for metabolic genes, flux balance analysis of the metabolic reconstruction can predict the essentiality of groups of genes. In Targeted gene removal experiments, the individual gene essentiality results are expanded to include the assignment of essentiality to combinations of genes in silico, leading to potential combinations of genes to remove to further minimize the genome. The classifications of the genes used in the metabolic reconstruction are shown in Table 2.

    Preliminary triple knockout experiments involving various sets of non-essential genes lead to cells with greatly impaired growth rates (data not shown). The fact that ∼15% of the genes in JCVI-syn3A are individually non-essential is not inconsistent with the near-minimality of the genome as a whole: it is not possible to remove all non-essential genes without vastly decreasing the growth rate or outright killing the cell. Furthermore, a genome comprised only of essential and quasi-essential genes is non-viable as well, since the removal of a non-essential gene can cause a previously quasi-essential gene to become non-essential in the new construct. As JCVI-syn3A grows more slowly than JCVI-syn (2 hr doubling time vs. 1 hr), a gene disruption that in JCVI-syn led to outgrowth by unaffected competitor cells might still survive through passage four in JCVI-syn3A. As a result, genes that were classified as quasi-essential in JCVI-syn can appear non-essential in JCVI-syn3A, and could in principle be removed as well—for the price of some gradual further decrease in growth rate. This lack of a clear cutoff again underscores the ‘trade-off between genome size and growth rate’ taking place during genome minimization (Hutchison et al., a).

    Biomass composition and reaction

    The cellular components of JCVI-syn3A fall into three categories: macromolecules, lipids and capsule, and small molecules and ions. Appendix 1—table 1 lists the mass fractions for all components included in the JCVI-syn3A biomass composition. These mass fractions are used to derive the coefficients in the biomass reaction depicted in Figure 4 for each component based on its molecular weight. The different biomass components are summarized below with the full discussion and derivation in Appendix 1. The growth-associated maintenance (GAM) ATP cost shown in Figure 4 is described in Section 'GAM/NGAM'.

    Macromolecules

    The macromolecular mass fractions are based on the experimental composition of M. mycoides capri (Razin et al., ), which is assumed to provide a very good approximation for JCVI-syn3A (which was derived from an M. mycoides capri substrain). The DNA fraction is slightly increased from the reported 5% to %, which corresponds to exactly one chromosome in JCVI-syn3A. Assuming almost all RNA to be present as ribosomal RNA (rRNA) and around bases per ribosome yields an upper limit of ∼ ribosomes per average cell. This number would correspond to ∼20, ribosomes in E. coli when scaled by cell volume, which is within the observed growth dependent range of –73, for E. coli (Bremer and Dennis, ). The RNA base composition is based upon the close relative M. mycoides capri serovar LC (M. mycoides capri

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